Bio-Image Acquisition and Processing Facilities,

1
Bio-Image Acquisition and Processing
Facilities, from Molecules to Brain
2
(No Transcript)
3
(No Transcript)
4
Bugnon 7-9
Fixed
Dynamic
Atomic Force
Confocal
Electron
Microscope
microscope
Microscope
(
nanoM
)
(µM)
(
nanoM
)
5
(No Transcript)
6
Program Les formats de stockage des images
numériques R. Kraftsik Fluorescence imaging in
living cells J. Staple Dynamic measurements of
intracellular sodium in living astrocytes
Jean-Yves Chatton Time lapse video of mobile
cells (Dictyostelium) C. Reymond Microscopie
holographique à contraste de phase quantitative
dans les cellules vivantes P. Marquet Visualizat
ion of molecules using the atomic force
microscope S. Kasas Visualisation des images
confocales en 3D avec le logiciel Imaris N.
Garin Quantification des structures 3D à partir
de coupes histologiques sériées R.
Kraftsik. Analyse morphométrique des arbres
neuronales et simulation L. Tettoni Biomedical
imaging S. Clarke Autoradiographie avec NIH
Image G. Bronchti Analysis of gels and blots
using Bioprint and 1D program B. Riederer
7
(No Transcript)
8
Les formats de stockage des images numériques

• R. Kraftsik

9
(No Transcript)
10
(No Transcript)
11
(No Transcript)
12
http//www.matisse.net/files/formats.html
http//www.matisse.net/files/glossary.html
13
BMP BMP is the standard Windows bitmap image
format on DOS and Windows-compatible computers.
When saving an image in this format, you can
specify either Microsoft Windows or OS/2 format
and a 1-bit to 24-bit depth for the image. For
4-bit and 8-bit images, you can also choose to
use Run-Length-Encoding (RLE) compression this
compression scheme is lossless, that is, it does
not discard detail from the image.
14
Photoshop EPS The Encapsulated PostScript (EPS)
language file format is supported by most
illustration and page-layout programs, and in
most cases is the preferred format for these
applications. Note that Photoshop EPS also
supports transparent whites in Bitmap mode. For
more information on EPS options, see Saving files
in Photoshop EPS format.
15
EPS TIFF or EPS PICT Preview You can use these
formats to open files saved in applications that
create previews but are not supported by Adobe
Photoshop (such as QuarkXPress). An opened
preview file can be edited and used like any
other low-resolution file.
16
CompuServe GIF The CompuServe Graphics
Interchange Format (GIF) is the file format
commonly used to display indexed-color graphics
and images in hypertext markup language (HTML)
documents over the World Wide Web and other
online services. GIF is a compressed format that
is designed to minimize file transfer time over
phone lines. Adobe Photoshop 4.0 can save Bitmap
mode, grayscale, or indexed-color images in the
CompuServe GIF format. When saving an image as
GIF, you can specify how the image appears as it
is downloaded. Select Interlaced to display the
image gradually in increasing detail as it is
downloaded. The GIF89a Export command lets you
specify the appearance of transparent areas in
the image and save an RGB image in the GIF
format. For more information, see Exporting
images.
17
JPEG The Joint Photographic Experts Group (JPEG)
format is commonly used to display photographs
and other continuous-tone images in hypertext
markup language (HTML) documents over the World
Wide Web and other online services. Unlike the
GIF format, JPEG retains all the color
information in an RGB image. JPEG also uses a
compression scheme that effectively reduces file
size by identifying and discarding extra data not
essential to the display of the image. Opening a
JPEG image automatically decompresses
it. Because it discards data, the JPEG
compression scheme is referred to as lossy. This
means that once an image has been compressed and
then decompressed, it will not be identical to
the original image. A higher level of compression
results in lower image quality, while a lower
level of compression results in better image
quality. In most cases, compressing an image
using the Maximum quality option produces a
result that is indistinguishable from the
original. For information on choosing JPEG
options, see Saving files in JPEG format.
18
PDF The PDF format is used by Adobe Acrobat,
Adobes electronic publishing software for
Macintosh, Windows, UNIX, and DOS. You can view
PDF files using the Acrobat Reader software
included on your Adobe Photoshop CD-ROM. Based on
the PostScript Level 2 language, PDF can
represent both vector and bitmap graphics. For
the purposes of representing pages, PDF pages are
identical to PostScript pages, but PDF files can
also contain electronic document search and
navigation features. PDF files, for example, can
contain hypertext links and an electronic table
of contents For more information on PDF and
Adobe Acrobat see the Electronic Publishing Guide
included on the Adobe Photoshop Tutorial CD-ROM.
19
PICT File The PICT format is widely used among
Macintosh graphics and page-layout applications
as an intermediary file format for transferring
files between applications. The PICT format is
especially effective at compressing images that
contain large areas of solid color. This
compression can be dramatic for alpha channels,
which often consist of large areas of white and
black. When saving an RGB image in PICT format,
you can choose either a 16-bit or 32-bit pixel
resolution. For a grayscale image, you can choose
from 2, 4, or 8 bits per pixel. If youre using a
Macintosh with QuickTime installed, you can also
choose from four JPEG compression options for the
file.
20
PNG The PNG format was developed as an
alternative to the GIF format and, like GIF, is
used for displaying images on the World Wide Web
and other online services. PNG preserves all
color information and alpha channels in an image
and uses a lossless compression scheme to reduce
file size. When saving an image in PNG format,
you can choose to display the image in gradually
increasing detail as it is downloaded. To do
this, select Adam7 for Interlace. You can also
select a filtering algorithm, which is used to
prepare the image data for compression.
21
TIFF The Tagged-Image File Format (TIFF) is used
to exchange files between applications and
computer platforms. The TIFF format supports LZW
compression, a lossless compression method that
does not discard detail from the image. When you
save an Adobe Photoshop image in TIFF format, you
can choose to save in a format that can be read
either by Macintosh or by IBM PC-compatible
computers. You can also choose to compress the
file to a smaller size automatically by clicking
the LZW Compression check box. Adobe Photoshop
reads and saves captions in TIFF files. This
feature is of particular use with the Associated
Press Picture Desk system, which uses the same
TIFF caption fields. For information on using
captions, see Adding file information.
22
(No Transcript)
23
TIFF 2077 KB 982X720707040 707Kpixel
24
JPEG 135 KB 707Kpixel