iGEM TEAM EPF-LAUSANNE

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iGEM TEAM EPF-LAUSANNE
International Genetically Engineered Machine
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OUR TEAM OUR SCHOOL
Carine
Christian
Tu
Nathalie
Nicolas
Rafael
Nicolas
Gabriela
10 undergraduate students 6 advisors
Heidi
Sebastian
Caroline
Mélanie
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Specific Aims 1. To develop strategies to
regulate gene expression using light as a
stimulus.2. To use computational tools to
optimize the selected strategies.
Our approach To utilize defined molecular
systems involved in gene regulation.The
activity of the molecules involved should be
amenable to regulation by light-induced changes
in protein conformation.
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Gene Regulation by Modulating Protein
Conformational Changes

• The LovTAP system

• Features
• Light-activated
• Reversible
• Synthetic system

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Gene Regulation by Modulating Protein
Conformational Changes
Experimental Design
RO1
RO2
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Experimental Design iGEM TOOLS
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Experimental Design Cloning Strategy
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Results Read-out n1 characterization
RO1 without Tryptophan
RO1 with Tryptopan
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Results Entire system (LovTAP read-out n2)
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Specific Aim 2 To use computational tools to
optimize the selected strategies

• The LovTAP system

• Desired Features
• Increased lifetime of the light-activated
  protein
• Enhanced affinity of the binding of the
  light-activated protein to the DNA

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Experimental Design Insight from Molecular
Dynamics and Biochemical Modeling
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Applications

• Specific and temporal induction of gene
  transcription in mammalian cells
• Coupling with gene recombination systems

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Acknowledgements

• Sebastian Maerkl Laboratory of Biological
  Network Characterization
• Bart Deplancke Laboratory of Systems Biology
  and Genetics
• Matteo Dal Peraro Laboratory for Biomolecular
  Modeling
• Felix Naef Computational systems biology

Thank You For Your Attention