Publications By

1
PublicationsBy

• AMANY I. HAMZA
• Assistant Professor of Parasitology
• Faculty of Medicine
• Assiut University

2
Research No. (9) Co-author

• EVALUATION OF SEROLOGICAL TESTS IN DIAGNOSIS OF
  TOXOPLASMOSIS AMONG COMPLICATED PREGNANCY

3
This work was conducted on 100 pregnant
women with previous complicated obstetric history
suggesting Toxoplasma infection as abortion,
stillbirth, neonatal death or babies with
congenital anomaly.

• Blood samples were collected from all cases and
  sera were separated and preserved at 20 ºC.
• Anti-Toxoplasma IgG and IgM antibodies were
  evaluated using four commercial serological
  tests

4

•   Latex agglutination test
• The test was done using the reagent (SAS
  TOXO RAPID LATEX, SAS scientific Inc.,Texas,
  USA).
• It was used for detection of Toxoplasma IgG
  antibodies.
•   Indirect haemagglutination test (IHAT)
• The test was done using TOXO HAIFUMOUZE
  (Laboratories FUMOUZE division diagnostics,
  Levallois Pepret, France).
• It was used for detection of Toxoplasma IgG
  antibodies.

5

• Enzyme linked immunosorbent assay (ELISA)
• ELISA is taken as the standard serological test
  that is going to be evaluated with the other
  serological tests. It was done using the
  commercial kit TOXOPLASMA GONDII IgG IgM ELISA,
  NOVUM DIAGNOSTICA GMBH, Germany.
• It was used for determination of IgG and IgM
  antibodies to Toxoplasma gondii in human serum.  
•  Immuno-Comb test
• The test was done using Immuno-comb TOXO IgG
  IgM, obtained from PBS (Organics, Courbevoie
  cedex, France).
• It was used for the determination of IgG and
  IgM antibodies to Toxoplasma gondii in human
  serum.

6
Results Serodiagnosis of Toxoplasma gondii
(IgG) antibodies in (100) pregnant women by
Latex, IHAT, ELISA and Immuno-comb
7
Serodiagnosis of Toxoplasma gondii (IgM) in (100)
pregnant women using ELISA and ImmunoComb
8
Correlation of Seropositivity of Toxoplasma (IgG)
and (IgM) antibodies in (100) pregnant women
using ELISA
9
Correlation of Seropositivity of Toxoplasma
(IgG) and (IgM) antibodies in (100) pregnant
women using Immuno-Comb
10
Qualitative agreement of the Latex and ELISA
(IgG) in (100) pregnant women with complicated
obstetric history

• Sensitivity of Latex test 95.2.
• Specificity of Latex test 83.7.
• Qualitative agreement 91.

11
Qualitative agreement between IHAT and ELISA
(IgG) in (100) pregnant women with complicated
obstetric history

• Sensitivity of IHAT 93
• Specificity of IHAT 76.7
• Qualitative agreement 86.

12
Qualitative agreement between ImmunoComb and
ELISA (IgG) in (100) pregnant women with
complicated obstetric history

• ? Sensitivity of Immuno-Comb test 100
• ? Specificity of Immuno-Comb test 100
• ? Qualitative agreement 100.

13
Qualitative agreement between ImmunoComb and
ELISA (IgM) in (100) pregnant women with
complicated obstetric history

• Sensitivity of Immuno-Comb test 100
• Specificity of Immuno-Comb test 93.6
• ? Qualitative agreement 97.
•  

14
Conclusion

• All the tests used in this study revealed a
  significant correlation, and that combination of
  more than one test can be recommended as a
  satisfactory and economic procedure for
  establishing the diagnosis of toxoplasmosis.
• The high prevalence of toxoplasmosis among
  tested women indicated that Toxoplasma infection
  is an important cause of complicated pregnancies.
  This constitutes an important health problem for
  pregnant women and neonates