F 2006 BIOC 3405

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F 2006 BIOC 3405

• 11-16-06

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Nucleotides

• Nucleoside phosphate
• Base and ribose (or deoxyribose)
• Pyrimidine
• Purine

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Minor Bases

• Nomenclature
• Substituent on ring
• Substituent on N on ring

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Reason for instability of RNA
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Bases

• Slightly basic
• Highly conjugated
• UV absorbers
• Nearly flat (Purines slightly out of plane)
• Insoluble at normal pH
• Hydrophobic stacking minimizes interaction with
  water
• Capable of H-bond formations in complementary ways

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Schreiner U, 1977-1990
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Chargaffs Rules

• DNA from different species have different base
  compositions
• DNA from same species (or tissues from same
  individual) have the same composition
• Base composition constant over time,
  environmental conditions, state of organism
• AT and GC

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