iGEM 2005

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iGEM 2005 2006
http//arep.med.harvard.edu/SBP/iGEM/iGEM_2004.ppt
http//bio.freelogy.org/wiki/IGEM2005 http//open
wetware.org/wiki/IGEMHarvard/2006 http//openwetw
are.org/wiki/IGEMHarvard/2007
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2007 Pre-iGEM meetings

• Overview
• Parts Devices
• Case study ( Voigt review)
• New Strengths
• 2 list of working parts systems
• 3. Brainstorm projects
• 4. Show how to design order parts.

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Biobricks
Proposal that the 2007 iGEM awards be biological,
useful, CO2-neutral.
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The 2004 iGEM poster was synthetic, but not
biological
( an artifact of light sensitive dyes)
Privately rehabilitated in time for 2005 iGEM
jamboree then publication Levskaya A, et al.
Nature 2005 Nov 24 Synthetic biology
engineering E.coli to see light.
Low contrast Input Red light /
phycocyanobilin Out LacZ / S-gal
(3,4-cyclohexenoesculetin- -D-galactopyranoside)
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Light Input/Output
Voigt 2006 Current Opinion in Biotechnology
2006, 17548557
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Possible alternatives for light I/O

• Caged substrates for light input
• Multiple wavelengths
• Luciferase output

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Potential strengths barely tapped

• Combinatorial constructions
• Selections
• Surface expression
• Homologous recombination

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