Gene Transcription

1
Gene Transcription
What is needed to transfer genetic information
encoded in the DNA into RNA?
DNA template

• DNA template
• RNA polymerase
• Ribonucleotides

RNA polymerase
Ribonucleotides
RNA polymer
2
Initiating Gene Transcription
How does RNA polymerase know where to start?
3
Basal Promoter
other regulatory regions
4
Basal Promoter
Shortening the region between the TATA box and
original start site shifted the beginning point
of transcription downstream.
5
Preinitiation Complex Assembly
TFII s TATA Binding Protein Complex
6
Gene Transcription
7
Gene Transcription
8
Promoters and Enhancers
Promoters are the minimum DNA sequences needed
for basal transcription and enhancers are DNA
sequences involved with the alteration the basal
transcription rate.
9
Postranscriptional Processing
Primary transcript
10
Postranscriptional Processing