Purpose of Stains

1
Purpose of Stains

• Simple Stain
• Negative Stain
• Gram Stain

• Morphology
• Morphology
• Classification

2
Results of Stains

• Simple Stain
• Negative Stain
• Gram Stain

• Blue bacteria
• Red background white bacteria
• bacteria purple - bacteria pink

3
Simple Stain Broth

• 7.
• 2.
• 3.
• 5.
• 6.
• 1.
• 4.

• Observe
• Using A.T. transfer culture to slide
• Air dry
• Stain 60 seconds
• Rinse and blot dry
• Clean slide
• Fix by passing through flame

4
Simple Stain Slant

• 8.
• 3.
• 4.
• 6.
• 7.
• 1.
• 5.
• 2.

• Observe
• Using A.T. transfer culture to slide
• Air dry
• Stain 60 seconds
• Rinse and blot dry
• Clean slide
• Fix by passing through flame
• Add 1 drop of water to slide

5
Negative Stain

• 2.
• 4.
• 6.
• 5.
• 3.
• 1.

• Place stain on slide
• Air dry
• Observe
• Fix by passing through flame
• Using A.T. place culture on slide
• Clean slide

6
Gram Stain

• 7.
• 6.
• 5.
• 3.
• 10.
• 1.
• 8.
• 2.
• 4.
• 9.

• Blot
• Place in Alcohol
• Rinse and Blot dry
• Rinse immediately
• Observe
• Using A.T. prepare a slide and fix
• Add Safranin O 20 seconds
• Add Crystal violet for 60 seconds
• Add iodine for 60 seconds
• Rinse and Blot dry