Yeast Transformation

1
Yeast Transformation

• Mod 2 Day 2
• Thursday Oct 9th

2
A little more about your yeast

• NY411
• MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52,
  trp1d63

3
A little more about your yeast

• NY411
• MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52,
  trp1d63

Ty917
4
A little more about your yeast

• NY411
• MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52,
  trp1d63

5
Transformation

• Wash the cells with Wash Soln ( water?)
• Resuspend the cells in Competent Soln ( LiAc?)
• Mix with your DNA samples
• No DNA, pRS414, concentrated/cleaned PCR
• Resuspend in Transformation Soln ( PEG?)
• 1 hr, 30
• Plate on SC-trp

6
Select TRP transformants
7
For next time.

• Work on your materials and methods to this point
• Read article for discussion in lab
• Revise Mod1 report if you want
• Enjoy the fall weekendyouve all been working SO
  hard