Blue Baroque

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Blue Baroque
A performance-art piece by David Mason,
interpreted by G. Prody. Friday May 10, 8 pm,
Bellingham Unitarian Fellowship.
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Reading Alberts pp. 198-232
262-266 MVA pp.1068-1086, 1093-1096 problems
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VVP Fig. 23-2
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VVP Fig 23-2
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MVA Fig. 28.1
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MVA Fig. 28.7 A mitotic chromosome
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Alberts, Figure 4-23
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MVA Fig. 28.11
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prolate ellipsoid 100 x 70 Angstroms.
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Properties of histones
residues MW arg lys
H1 215 23.0 1 29 H2a 129 14 9 11 H2b 125 13.8 6 16
H3 135 15.3 13 10 H4 102 11.3 14 11
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VVP Fig. 23-44b
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Alberts Figure 4-28
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MVA Fig. 28.10
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VVP Fig. 23-45
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Alberts Figure 4-30
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MVA Fig. 28.13
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MVA Fig. 28.12
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MVA Fig. 28.17
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MVA Fig. 28.19 Telomerase
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VVP Fig. 27-31 Chromosome Puffs where
transcription is occurring.
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MVA Fig. 28.27
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Demonstration that transcriptionally active genes
are more susceptible than inactive genes to DNase
I digestion. Chick embryo erythroblasts at 14
days actively synthesize globin, whereas
cultured undifferentiated MSB cells do not.
Nuclei from each type of cell were isolated and
exposed to increasing concentrations of DNase I.
The nuclear DNA was then extracted and treated
with the restriction enzyme BamHI, which cleaves
the DNA around the globin sequence and normally
releases a 4.6-kb globin fragment (a). The DNase
I- and BamHI-digested DNA was subjected to
Southern-blot analysis with a probe of labeled
cloned adult globin DNA, which hybridizes to the
4.6-kb BamHI fragment. As shown in (b) the
transcriptionally active DNA from the 14-day
globin-synthesizing cells was sensitive to DNase
I digestion, indicated by the absence of the
4.6-kb band at higher nuclease concentrations. In
contrast, the inactive DNA from MSB cells was
resistant to digestion. See J. Stalder et al.,
1980, Cell 19973.
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Alberts Fig. 4-35
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Alberts Fig. 4-35
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MVA Fig. 28.28 Acetylation of histone cores on
lysines.
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Experimental method for analyzing the
acetylation state of histones in chromatin
associated with a specific region of the genome.
Nucleosomes are lightly cross-linked to DNA in
vivo using a cell-permeable, reversible, chemical
cross-linking agent. Chromatin is then isolated,
sheared to an average length of three
nucleosomes, and subjected to immuno-precipitation
with an antibody specific for a particular
acetylated N-terminal histone sequence. The DNA
in the immunoprecipitated chromatin fragment is
released by reversing the cross-link and then is
quantitated using a sensitive PCR method. See S.
E. Rundlett et al., 1998, Nature 392831.


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MVA Fig. 28.29 Polyadenylation