Advanced Tissue Engineering Tissue Engineering III

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Advanced Tissue Engineering Tissue Engineering
III

• Spring 2005

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Advanced Tissue Engineering III, Spring 2005

• Objectives To provide graduate and advanced
  (senior) undergraduate biomedical
  engineering/science students with more detailede
  knowledge of (factor-mediated) tissue
  engineering
• Course length 11 weeks
• Prerequisite Courses TE I and TE II
• Venue/Dates
• New College Building 19th floor Conference Room
• Wednesdays 1200 - 200

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Advanced Tissue Engineering III, Spring 2005

• Textbooks
• No suitable text book available (yet). Will use
  select chapters from
• Lanza et al. Principles of Tissue Engineering,
  2nd edition, Acad. Press, 2000
• Lanza et al Principles of Tissue Engineering,
  1st edition, Acad. Press, 1997
• Palsson and Bhatia Tissue Engineering, Pearson
  Prentice Hall, 2003
• Saltzmann Tissue Engineering, Oxford University
  Press, 2004
• Dee, Kay C., Puelo Avid A., and Bizios Rena An
  Introduction to Tissue-Biomaterials Interactions,
  Wiley Liss Hoboken, NY, 2002.
• Elçin Y. Murat (ed) Tissue Engineering, Stem
  Cells, and Gene Therapies. Advances in
  Experimental Medicine and Biology vol 534. Kluwer
  Academic/ Plenum Press, New York, NY, 2003.

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Advanced Tissue Engineering III, Spring 2005
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Tissue Engineering Engineering Principles for the
Design of Replacement Organs and Tissues W.
Mark Saltzman Oxford Universitry Press, July 2004
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Advanced Tissue Engineering III, Spring 2005

• Other Relevant Textbooks
• Tozeren and Byers New Biology for Engineers
  Computer ScientistsPrentice Hall  2003 
• Alberts et al., Molecular Biology of THE CELL 4th
  edition., Garland, 2002 ()
• Lodish et al. Molecular Cell Biology, 4th
  edition, Freeman, 2000()
• Gilbert Developmental Biology, 6th edition,
  Sinauer, 2000 ()
• Karp Cell and Molecular Biology 2nd edition,
  Wiley 1999
• Cooper Cell, a Molecular Approach, Sinauer ,
  1997
• Alberts et al. Essential Cell Biology, Garland,
  1998
• Pollack Cells, Gels and Engines of Life, Ebner
  and Sons, 2001
• ()Avaiable on line http//www.ncbi.nlm.nih.gov/en
  trez/query.fcgi?dbBooks

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Advanced Tissue Engineering III, Spring 2005

• Week 1 Introduction Tools of the Trade
• Cells and their Interaction with their
  Environement (Factors)
• Week 2 Cells, Scaffolds, Bioreactors
• Week 3 Stem Cells and their Differentiation in
  vivo and in vitro
• Week 4 Factors I Biochemical Factors
• Week 5 Factors II Mechanical Factors
  I-Scaffolds
• Week 6 Factors III Mechanical Factors
  II-Hemodynamic Factors
• Mid-Term Examination Review Paper (2 weeks)
• pilelkes_at_drexel.edu lelkes 123_at_AOL.COM

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Advanced Tissue Engineering III, Spring 2005

• Week 7 Cardiovascular Tissue Engineering
  (Midterm due) Blood Vessels
• Week 8 Cardiac Tissue Engineering Hearts and
  their Parts Models
• Week 9 Skeletomuscular Tissue Engineering Bone,
  Cartilage and Tendon
• Week 10 Tissue Engineering of Visceral Organs
  Liver, Pancreas
• Week 11 Latest Developments in TE Stem Cell
  Therapy, Organ Printing, Ethics
• Final Examination Research Proposal (5 weeks)

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Advanced Tissue Engineering III, Spring 2005

• Mid-Term (Review) Paper
• Topics assigned 2 week before midterms , based
  on first 5 lectures Format J. Cell Biol
• Undergraduate Students
• (SENIORS)
• 10 -12 pages, double spaced, 1 margins, 11 pt
  TNR, min 10 references
• Graduate Students
• 18 - 20 pages , double spaced, 1 margins, 11 pt
  TNR, min 20 references

• Final Examination - Research Proposal
• Topics will be assigned after midterm
• Format NIH proposal
• Undergraduate Students (SENIORS)
• 18 - 20 pages, double spaced, 1 margins, 11 pt
  TNR min 15 references
• Graduate Students
• 30 - 35 pages, double spaced, 1 margins, 11 pt
  TNR min 30 references

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Advanced Tissue Engineering III, Spring 2005

• Peer Review System
• Mid-Term
• Papers due May 1, 2005
• Reviews due May 6, 2005
• 2. Finals
• Papers due June 5, 2005
• Reviews due June 10, 2005

• Example for Peer-Review System
• Authors Peer Reviewers
• Paper/Proposal Midterm Final
• 1 3 5
• 2 4 6
• 3 5 7
• 4 6 1
• 5 7 2
• 6 1 3
• 7 2 4

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Advanced Tissue Engineering III, Spring 2005

• Grading
• 10 Participation in Class
• 20 Journal Club/Presentation
• 30 Midterm Paper ( peer-review)
• 40 Final Research Proposal ( peer-review)

• Grades
• A 100 - 95 outstanding
• (extra effort, undergraduates at graduate level)
• A/B 95 - 90 very good
• B 90 - 80 good
• B/C 80 -75 average
• C 75 -70 fair,
• GRADS pass
• D 70 65 so la la,
• UNDERGRADS pass
• GRADS fail
• ltD FAIL

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Advanced Tissue Engineering III, Spring 2005

• Course Policies
• General Policies
• in accordance with university regulations
• Attendance
• Mandatory for both lecture and recitations,
  sign-up sheets
• Exams
• Midterm and Final Exams papers/proposals and
  peer-reviews due on dates stated
• Team papers with individual contributions clearly
  marked
• Students will be graded based on individual
  performance and as team members and as
  (individual) peer-reviewers
• Grading Disputes
• Must be settled with instructor within 1 week
  after grading

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Advanced Tissue Sciences and Engineering

• http//www.advancedtissue.com/
• You have reached the web site for the ATS
  Liquidating Trust.
• We intend for this site to be a continuing source
  of news and information on the activities of the
  Trust.

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• Tissue engineering demonstrates enormous
  potential for improving human health.
• While there is an extensive body of literature
  discussing the state of the art of tissue
  engineering, the majority of this literature is
  descriptive and does little to address the
  principles that govern the success or failure of
  an engineered tissue.
• This course will explore principles of tissue
  engineering, drawing upon diverse fields such as
  developmental biology, immunology, cell biology,
  physiology, transport phenomena, material
  science, and polymer chemistry.
• Current and developing methods of tissue
  engineering as well as specific applications will
  be discussed in the context of these principles.

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Tissue Engineering

• Biotechnological Platform for
• Enhanced Drug Screening
• Food Production
• Art

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Principles in Tissue EngineeringUNC

• The course will include a detailed knowledge of
  representative tissues and their structures and
  function, biomaterials used to fabricate tissue
  engineered devices, fabrication methods, cell
  culture and cell growth conditions, immunology
  and host responses to foreign bodies, matrix
  anabolism and catabolism, host-device
  interfacing, microelectronics, biotechnology
  development, intellectual property, animal model
  testing, human testing good laboratory practices,
  FDA regulations.
• At least one plant trip to a device or
  fabrication plant is planned.
• Guest lecturers, expert in the field, will give
  lectures.
• The course will include 3 one hour lectures per
  week or the equivalent. Grades will be computed
  based on results of two exams, a paper, quizzes,
  attendance and a final exam

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Tissue Engineering

• Biotechnological Platform for Enhanced Drug
  Screening

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Tissue Engineering and Art

• http//www.tca.uwa.edu.au/
• http//www.tca.uwa.edu.au/ars/timebased/1.html
• http//www.tca.uwa.edu.au/ars/timebased/2.html

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Advanced Tissue Engineering ?

• Growing a full scale human ear using tissue
  engineering techniques is still somewhat
  problematic.
• The engineered cartilage cells seem to loose
  their structural integrity and the whole form
  tend to collapse.

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Some Relevant URLs

• Cell/tissue culture automation
• http//www.automationpartnership.com/a/robotic-cel
  l-culture-gp.htm
• Search for tissue engineering
• http//www.dmoz.org/,
• http//search.dmoz.org/cgi-bin/search?search22ti
  ssueengineering22
• Art and Tissue Culture/Engineering
• http//www.tca.uwa.edu.au/
• http//www.symbiotica.uwa.edu.au/
• Image analysis for TE
• http//www.tissueinformatics.com/
• Vacanti-Lab MIT/Harvard
• http//www.mgh.harvard.edu/depts/tissue/projects/m
  ains/amain.html