Evolutionary Genomics of

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Evolutionary Genomics of Cytochrome P450
David R. Nelson University of
Tennessee Dept. of Molecular Sciences Center
of Excellence in Genomics and Bioinformatics
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1107
1547
469
483
205
P450 distribution in major groups of organisms
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469 163 Sargasso Sea seqs.
1547
55
488
1107
80
70
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Tree of Life
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Phytophthora
ramorum
Thalassiosira pseudonana
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Magnaporthe grisea
Fusarium graminearum
Neurospora crassa
Saccharomyces cerevisiae
Schizosaccharomyces pombe
CYP61
Phanerochaete (white rot)
Fungi
X
Aspergillus nidulans
Metazoa
X
Homo, Drosophila, C. elegans
mouse, rat, Fugu, Anopheles
Amoebozoa
X
Dictyostelium discoideum
(Slime molds)
Eukaryotic Crown Group
Stramenopiles
X
Diatoms, Kelp, Phytophthora
(Heterokonts)
- CYP51
Ciliophora
Tetrahymena, Paramecium
X
Alveolates
Apicomplexa
Plasmodium (malaria)
X
Toxoplasma
(animal parasites)
CYP51
CYP97
Viridiplantae
X
Arabidopsis, Oryza (rice)
Chlamydomonas, Poplar
Rhodophyta
X
Cyanidioschyzon, Porphyra
Plants
Glaucophyta
Cyanophora
X
Euglenozoa
Trypanosoma, Leishmania
Discicristata
Heterolobosea
Naegleria, Acrasis
Diplomonadida
Giardia lamblia
Archaezoa
Parabasala
Trichomonas
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CYP21
11A1
11-Deoxycorticosterone
11B1
Corticosterone
CYP17
11B2
CYP17
Aldosterone
CYP21
CYP17
CYP17
11-Deoxdycortisol
11B1
CYP19
Cortisol
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Ecdysone metabolic pathway in insects
From Niwa et al. J Biol Chem. Aug. 20 279,
35942-9 2004
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Brassinosteroid pathway
CYP90D
CYP90D
CYP90C
ROT3
CYP85A2
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P450 CLANS in plants

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• 71 71, 73, 75, 76, 77, 78, 79, 80, 81, 82,
  83, 84, 89, 92, 93, 97
• 98, 99, 701, 703, 705, 706, 712, 719,
  723, 726, 736
• 72, 709, 714, 715, 721, 734, 735
• 74
• 85, 87, 88, 90, 702, 707, 708, 716, 718, 720,
  722, 724, 725,
• 728, 729, 733
• 86, 94, 96, 704, 730, 731, 732
• 97
• 710
• 711
• 727 727

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P450 distribution in plants, 1100 sequences in 62
families plus 550 Populus
(cottonwood tree) P450s
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Collaborators Rice/Arabidopsis Mary Schuler
University of Illinois Soren Bak
Copenhagen Suzanne Paquette University of
Washington/ Copenhagen Daniele Werck-Reichhart
Strasbourg
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freshwater Hydra
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Halocynthia roretzi adult amd larva
Cnidarian
Chordate
Porifera (sponges)
Suberites domuncula
plus hermit crab
leech (annelid worm)
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GATA-1 promoter/GFP transgenic zebrafish
Buffer injected embryo under fluorescence
CYP20 Morpholino injected under fluorescence
CYP20 Morpholino injected under white light
Data of Todd Penberthy, with permission
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X chromosome genes near the QTL peak
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Other significant QTLs that appeared in 2-5
experiments (see http//drnelson.utmem.edu/qtl.ma
p.html for details) Cyp8b1 a cis QTL (peak on
top of Cyp8b1) 5X Cyp17a1 has a QTL on Chr 16
at 23-38 Mb 5X Cyp2a4 has a QTL on Chr 2 at
103-116 Mb 5X Cyp2d9 has a QTL on Chr2 at
36-48 Mb 4X Cyp7b1 has a QTL on Chr 18
at 3-15 Mb 3X Cyp4v3 has a QTL on Chr 10
at 75-88 Mb 2X Cyp2c37 has a QTL on Chr 18
at 47-59 Mb 2X Cyp2c50 has a QTL on Chr 1 at
32-48 Mb 2X Cyp2s1 has a QTL on Chr 12 at
13-23 Mb 2X
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Fish human tree
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