Laboratory of Enzyme Chemistry

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Laboratory of Enzyme Chemistry
Pacific Institute of Bioorganic Chemistry FEB RAS
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1,31,6-?-D-Glucans - Laminarans
5 kDa 1,31,6 9010
5 kDa 1,31,6 6535
Soloveva T.F., Elyakova L.A., Zvyagintseva T.N.,
Yermak I.M. Marine Technol. Soc. 1996. 30 35
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1,31,6-?-D-Glucans - Laminarans
from Laminaria guryanovae
b
c
a
Molecular weight 5-16 kDa 1,31,6 982
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Fucoidans
FUCOIDAN from Fucus evanescens (1?3
1?4-a-L-fucan)
Molecular weight 20-40 kDa Fuc 80 Gal, Xyl
20 Fucose SO42- 10.8
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Fucoidans
FUCOIDAN from Fucus evanescens
desulphatation
Nonsulphated FUCOIDAN
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Fucoidans
FUCOIDAN from Laminaria cichorioides
(1?3-a-L- fucan)
Molecular weight 60-80 kDa Fuc 98 FucSO42-
11.7
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Polymannuronic acids
Sources Alaria fistulosa Fucus evanescens

• Molecular weight
• kDa - 30 kDa
• 20 kDa - 40 kDa

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Alginic acids
Sources Alaria fistulosa Fucus
evanescens Laminaria cichorioides Undaria
pinnatifida
Molecular weight 900 kDa 900 kDa 900 kDa 900 kDa
Ratio M and G-block 2.81 1.01 2.51 3.01
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Enzyme transformation of polysaccharides
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Enzymes' Set
Endo- and exo-1,3-?-D-glucanases (marine mollusks
and fungi ) Alginat lyases (marine mollusks and
bacteria) Fucoidanases (marine mollusks and
bacteria) Endo-1,6-?-D-glucanases (marine
mollusks) Cellulases (marine bacteria) ?- and
?-D-Glucosidases (marine mollusks and bacteria)
?- and ?-D-Galactosidases (marine bacteria) ?-
and ?-D-Mannosidases (marine mollusks and
bacteria) ?-L-Fucosidases (marine
mollusks) Sulfatases (marine mollusks)
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Marine invertebrates and seaweeds are the
valuable sources of the biologically active
polysaccharides, the enzymes with unique
specificity involved in their transformation and
inhibitors of these enzymes.
So, endo-1,3-?-D-glucanases of marine organisms,
found at first as hydrolases, catalyze three
reactions running simultaneously and practically
with almost equal efficiency.
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1,3-?-D-glucanase from Chlamys albidus
Translam
8-10 kDa
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Enzyme transformation of the FUCOIDAN
Fucoidans, highly sulfated polysaccharides of
brown algae, posses diverse biological
activities. The most interesting are antitumor,
anticoagulant, and antiviral activities, e.g.,
against HIV, hepatitis virus, and herpes virus.
For the last decade, the structure of these
polysaccharides has been extensively studied. A
close correlation between structural
characteristics of fucoidans and the taxonomy of
the corresponding brown algae was hypothesized
it is known that a-(1,3)-L-fucans are found in
Laminaria, whereas species of Fucus genus mainly
contain a-(1,3, 1,4)-L-fucans. Structure/
activity correlations for these polysaccharides
are poorly studied. Usually fucoidans have a high
d. p., so depolymerisation is needed for
medicinal applications. The enzymes degrading
polysaccharides are widely used in structural
studies, in studies of biological activities, and
in preparation of drugs.
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Enzyme transformation of the FUCOIDAN from Fucus
evanescens
The products of enzymatic cleavage of fucoidans
by fucoidanases
HMP highly molecular products obtained by
precipitation with 80 aqueous ethanol (in of
total amount of products),n degree of
polymerization,LMP low molecular products
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Enzyme transformation of the FUCOIDAN from Fucus
evanescens
The characteristics of low-molecular products of
cleavage of fucoidan from F. evanescens by
action of fucoidanase from hepatopancreas of L.
kurila and P. citrea ??? 3296
n degree of polymerization of products
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Enzyme transformation of the FUCOIDAN from Fucus
evanescens
The fucoidanases from the marine mollusk L.
kurila and the marine bacterium P. citrea ???
3296 have a similar specificity they catalyze
the predominant cleavage of a-(1?3)-glycosidic
bonds between fucose residues in the
polysaccharide. In contrast to fucoidanase from
L. kurila, the bacterial fucoidanase cleaves
fucoidan forming mainly di-, tri-, tetra-, and
pentafucooligosaccharides, whereas the action of
the basic form of fucoidanase from L. kurila
yields higher molecular weight products of 3-10
kDa. Probably, these differences are related to
structural peculiarities of active centers of
enzymes and the mechanism of action of the
enzymes on the polymer substrate.
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Biological activity of polysaccharides from brown
seaweed of the Russian Far East Seas and their
enzymatic transformation products
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Biological activity of polysaccharides from brown
seaweed of the Russian Far East Seas and their
enzymatic transformation products
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Fractions of 1?31?6-?-D-glucans with higher
molecular masses and more branched than initial
laminaran were found among the products of its
enzymatic transformation by endo-1,3-?-D-glucanase
. These fractions designated as Antivir" and
Translam are the products of the prominent
glucanosyl transferase ability of the enzyme and
its ability to catalyze synthesis not only
?-1?3-, but also ?-1?6-glucosyl links.
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FUCOLAM-C?

• Substance for
• Supplements
• Cosmetics
• Medicine

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Fucoidan
plus
Polymannuronic acid
?
Immunomodulator