Sharing the KOMP Resources

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Sharing the KOMP Resources

• Mutant Mouse Repositories

Clonal ES cell lines
NARRC January 2007
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History of Mouse Resource Support

• 1959 - NSF funded 1st JAX mouse repository
• 1978 - Division of Research Resources
• (pre-NCRR) funded the JAX mutant
• mouse repository
• 1999 - NCRR funded MMRRC to expand
• mouse resource capability
• 2006 - NIH funded
• Repatriation of targeted mutation strains
• Acquisition of Deltagen/Lexicon strains

NARRC January 2007
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Value of KOMP Resources

• Provide mutant mice and ES cells for
• Basic research to basic clinician researchers
• Understand pathways
• Understand gene function
• Identify drug targets
• Phase 0 drug trials
• Critical to archive and distribute knockout
  mouse resources

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Size of current NCRR resources
JAX C57BL/6J ES cell line distributed by ATCC.
TOTAL 2928 15,327 18,255
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Partnerships for Sharing Resources

• FIMRe - Federation of International Mouse
  Resources
• Coordinate mouse resource archiving and sharing
• Provide each other local distribution centers
• Standardize protocols
• Share advancements and training
• IMSR - International Mouse Strain Resource
• One-stop on-line shop for mouse resources
  available worldwide

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Maintenance of resources

• Living mice are not necessary - impossible except
  for strains in continuing demand
• Gametes ES cells are mice in different formats
• 2/3 of strains at JAX in frozen state only
• 96 of strains in MMRRC in frozen state only
• Both resource programs cryopreserve strains
  during the importation process
• C57BL/6 sperm can now be reliably frozen and
  recovered by IVF
• Frozen sperm enable recovery of large numbers of
  mice

NARRC January 2007
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