Biol 1020 Lab 2 - Biochemistry Lab Review

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Biol 1020Lab 2 - BiochemistryLab Review
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Question 1

• Which of the following would be found in starch?

A
B
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Question 1

• Which of the following would be found in starch?

This is a Glucose
A
B
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Question 2

• If the 2 glucose's below were to join where and
  how would the bond form?

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Question 2

• The bond would form between the 1st and 4th
  carbon. It would involve the removal of an OH
  and a H.

A
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Question 2

• The bond would form between the 1st and 4th
  carbon. It would involve the removal of an OH
  and a H.

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C
H

O
H

6
C
H

O
H
2
2
O
O

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H
H
H
H
A
H
H

4
1
1
O
H
O
H
H
H
O
H
H
O
O


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H
O
H
H
O
H
A new a 1-4 bond
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Question 3

• What is this type of reaction called?

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C
H

O
H

6
C
H

O
H
2
2
O
O

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H
H
H
H
A
H
H

4
1
1
O
H
O
H
H
H
O
H
H
O
O


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H
O
H
H
O
H
A new a 1-4 bond
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Question 3

• The reaction is a condensation reaction and
  involves the removal of water (H O).

2

6
C
H

O
H

6
C
H

O
H
2
2
O
O

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H
H
H
H
A
H
H

4
1
1
O
H
O
H
H
H
O
H
H
O
O


3
H
O
H
H
O
H
A new a 1-4 bond
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Question 4

• Is glucose in this form a reducing sugar?

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Question 4

• No- The double bonded Oxygen is part of the ring.
  We need to heat the solution to open the ring
  for the Benedicts test to be positive.

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Question 5

• Which of the Catechol temperatures reacted
  fastest?
• A. Ice bath
• B. Room Temp
• C. 100ºC
• D. 40ºC

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Question 5

• Which of the Catechol temperatures reacted
  fastest?
• A. Ice bath
• B. Room Temp
• C. 100ºC
• D. 40ºC

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Question 6

• Why did the 100ºC Catechol not react?
• A.The catechol bonds were broken by the heat
• B.The catachol oxidase bonds were broken by the
  heat
• C. The catachol oxidase was denatured by the heat
  
• D. The catechol was denatured by the heat

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Question 6

• Why did the 100ºC Catechol not react?
• A.The catechol bonds were broken by the heat
• B.The catachol oxidase bonds were broken by the
  heat
• C. The catachol oxidase was denatured by the heat
  
• D. The catechol was denatured by the heat

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Question 7

• Why did the 0ºC Catechol not react?
• A.The catechol was destroyed by the ice
• B.There was not enough energy for the reaction to
  occur
• C. The potato was frozen
• D. The catechol oxidase was destroyed by the ice

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Question 7

• Why did the 0ºC Catechol not react?
• A.The catechol was destroyed by the ice
• B.There was not enough energy for the reaction to
  occur
• C. The potato was frozen
• D. The catechol oxidase was destroyed by the ice

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Question 8

• Which of the following would you think are non-
  polar molecules based on the ability to mix with
  oil?
• A. Ether
• B. Water
• C. Soap

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Question 8

• Which of the following would you think are
  non-polar molecules based on the ability to mix
  with oil?
• A. Ether
• B. Water
• C. Soap
• The soap did not mix-it emulsified

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Question 9

• Which of the following is a difference between a
  fat and a phospholipid?
• A. fats have 3 fatty acids and phospholipids have
  2
• B. fats have non polar heads and phospholipids
  have polar heads
• C. fats are used as an energy store while
  phospholipids form bilayers (membranes)
• D. fats usually contain only H,C,O while
  phospholipid also contain N and P

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Question 9

• Which of the following is a difference between a
  fat and a phospholipid?
• A. fats have 3 fatty acids and phospholipids have
  2
• B. fats have non polar heads and phospholipids
  have polar heads
• C. fats are used as an energy store while
  phospholipids form bilayers (membranes)
• D. fats usually contain only H,C,O while
  phospholipid also contain N and P
• All are true

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Lab 2 - Biochemistry
Your lab bench must be cleaned up before you
leave. Place all of the test tubes back in the
rack! Leave all material in its original location
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Biol 1020Lab 2 - BiochemistryLab Review