Laboratory Eleven

1
Laboratory Eleven

• The Circulatory System
• Physiology

2
The Electrocardiogram Recording of Heart
Electrical Activity (p202, 11A Procedure, 2)

• P wave
• Atrial depolarization
• Occurs before atrial contraction
• Generated by the firing of Sinoarteial (SA) node
• QRS complex
• Ventricular depolarization
• Occurs before ventricular contraction (systole)
• T wave
• Ventricular repolarization
• Occurs before ventricular relaxation (diastole)

3
Pulse Blood Pressure(p203, 11B Procedure)

• Determine pulse rate manually in 5 different
  locations
• Measure systemic blood pressure using the
  peripheral pulses in the brachial artery
• Systolic pressure (first soft sound) generated
  by contraction of left ventricles
• Diastolic pressure (sound terminates)
  corresponds to relaxation of the left ventricle

4
Blood Typing
Anti-Serum Agglutination Antigen
Anti-A Yes A Present
Anti-A No A NOT Present
Anti-B Yes B Present
Anti-B No B NOT Present
Anti-Rh Yes Rh Present
Anti-Rh No Rh NOT Present
5
Blood Typing(p205, 11C Procedure)

• 2 drops of the synthetic blood of only one
  subject
• 2 drops of each anti-serum
• Mix with toothpicks (avoid cross contamination)
• Place the slide on the Rh-typing box for 30-45sec
  while gently rocking the box
• Observe the wells for agglutination (RBC
  clumping)
• Record your results in your lab report
• e.g.
• B- (agglutination in anti-B well)
• O (agglutination in anti-Rh well)
• AB (agglutination in Anti-A, -B, -Rh wells)

6
A Typical Antibody