Title: First a review
1(No Transcript)
2First a review
- Adopt as a perspective that
- One B-cell produces one antibody
- Make that perspective more exacting
- One B-cell produces one idiotype.
-
- Cassettes (V, J, D,) (aka gene segments) fuse
(to make an idiotype). - Fusing occurs because RAG recognizes one turn/two
turn signals on cassettes. - CDR3 develops through junctional flexibility
(trimming) and addition of P N nucleotides
(expanding). -
- CDR1 and CDR2 (especially) and CDR3 become more
varied because of somatic hypermutation.
3First a review now with images
- And, I will use images from a different source.
- Remember to be critical of what interpretative
drawings convey
4First a review now with images
5First a review still with images
- RAG what does it do? how does it work?
6First a review still with more images
- RAG what does it do? how does it work?
- what about PN nucleotides?
7PART IV (some new stuff)
- How do immunoglobulins assemble?
- Some immunoglobulins are in the surface membrane
of immature B-cells while other immunoglobulins
of the same idiotype are secreted by mature
B-cells. Whats the difference? - Similarly, identical variable regions can be
shared among different isotypes. How? - B-cells are diploids with two sets of genetic
instructions. How does just one set get
expressed?
8How do immunoglobulins assemble?
9Some immunoglobulins are in the surface membrane
of immature B-cells while other immunoglobulins
of the same idiotype are secreted by mature
B-cells. Whats the difference?
10Similarly, identical variable regions can be
shared among different isotypes. How?
11B-cells are diploids with two sets of genetic
instructions. How does just one set get
expressed?
12The general scheme of B-cell development
- STEM CELL
- ?
- pro (genitor) Bcell
- ?
- pre Bcell
- ?
- Ag-selected (? stimulated)- Bcell
- ?
- Ab secreting plasma cell