Uses of the CCD

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Uses of the CCD

• Image fluorescence - living or fixed, multiple
  colors
• Image phase or DIC - living or fixed

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Elements of the CCD workstation (located in Rm
012)

• Microscope
• CCD camera
• Computers for image aquisition/analysis
• Filter wheels
• Acquisition/analysis software- Openlab
• CO2 incubator and heating stage
• Focus drive

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Elements of the CCD workstationLeica DMIRB -
inverted microscope

• 20X phase - adjustable focal length - to be used
  with anything from thick petri plastic to thin
  (0.17um) coverslips
• 40X phase- adjustable focal length -
• 63X phase- adjustable focal length -
• 63X water immersion (no phase)
• Oil immersion objectives can be shared with the
  confocal

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Elements of the CCD workstationCCD camera

• Princeton Instruments MicroMax
• 12-16 bit, 1000X800 pixels
• Back illuminated, cooled CCD
• High resolution/high sensitivity

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Elements of the CCD workstationComputers for
image acquisition and analysis

• Acquisition computer (Mac G3, 256 MB RAM, 4GB) is
  located in room with camera - data should be
  transferred to CD or the analysis computer soon
  after acquisition because files are large.
• Analysis computer is located in Room 123

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Elements of the CCD workstationFilter Wheels

• Emission/Excitation Filter Wheels
• Filters Include
• FITC
• CY3/Texas Red
• GFP
• BFP

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Elements of the CCD workstationMicroscope-attache
d CO2 incubator and heating stage

• Allows long-term experiments at constant
  temperature and CO2

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Elements of the CCD workstationAcquisition and
Analysis Software - Openlab

• Macros control camera/focus drive/filter wheels
  for long-term acquisition
• Analysis package allows manipulation of images
  for presentation and quantitation

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Elements of the CCD workstationAcquisition and
Analysis Software - Openlab
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Elements of the CCD workstationFocus Drive

• Allows acquisition from multiple focal planes -
  can compensate for movement of living structures
• Can be used to obtain confocal images in
  conjunction with the Silicon Graphics program
  Imaris

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Elements of the CCD workstationFocus Drive
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