microRNAs Genomics

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microRNAs Genomics Rosetta Genomics
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miRNAs
Reference Bartel (Cell 2004) MicroRNAs genomics
biogenesis mechanism and function
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MicroRNAs Biogenesis

• Transcription by RNA polymerase as separate
  gene or in intron of coding protein gene.
• First step Drosha cleavage.
• Second step Dicer cleavege.
• Choosing strand, one mature miRNA is incorporate
  into RISC.

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miRNA genomic properties.

• Conserved
• Clustered

Reference UCSC genome browser.
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miRNA Function

• Development, differentiation, determining cell
  fate let7, lin4 major role in C. elegans
  development.
• lye6 - controlling left/right neuronal
  asymmetry in C. elegans
• mir-143 regulates Adipocyte differentiation.
• Tissue specific miRNAs Liver (mir-122), Brain
  (mir-124).
• Protein production control
• mir-375 regulates insulin secretion.
• Major role in many diseases.