Safety Procedures

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Safety Procedures

• Working with Laboratory Animals

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University Animal CareDepartment

• Faculty and Student Resources
• Personal Protective Equipment
• Technical Training and Project Support
• Guidance with and Handling of Protocol
  Submissions
• Laboratory Animal Occupational Health Program
  (LAOHP)

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Ethics of Working with Laboratory Animals

• Animal Use Protocols and IACUC Review
• Humane Care and Treatment of Animals
• Reporting Concerns

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Institutional Animal Care and Use Committee

• Must approve all vertebrate animal work before
  the project commences
• Adhere to government standards
• for protocol review
• Function and Composition

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PROTOCOL REVIEW PROCESS
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Protocol Review Criteria

• Humane Care and Use of Animals
• Reduction, Replacement, and Refinement
• Personnel Training and
• Experience
• Personnel Safety and
• Risk Assessment

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Working with Laboratory Animals

• No Eating, Drinking, Smoking
• Pen/Nail/Gum Chewing or
• Applying Cosmetics.
• Wash Hands Routinely
• Exercise UNIVERSAL PRECAUTION

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Working with Laboratory Animals

• Use Common Sense and Communicate
• EXPECT the Unexpected
• Be Mature, Professional
• and Respectful

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Personnel Training Resources

• Radiological Safety Training
• MRI Equipment Training
• Chemical Safety MSDS
• Biosafety Training
• Animal Care and Use Training

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Personal Protective Equipment

• Lab Coats or Scrub Attire
• Gloves and Gauntlets
• Masks and Respirators
• Goggles and Splash Guards
• Sterile Approach (Aseptic Technique)

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Lab Coats and Gloves

• Both should be worn when handling live animals or
  their tissues (BSL-1)
• Gloves to be removed at work station
• NEVER to be worn in the hallway or while using
  the telephone
• Shoe covers when working with isotopes

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Masks and Respirators

• Guards Against Zoonotic Airborne Diseases (e.g.,
  Hanta virus)
• Reduces exposure to Animal Allergens
• Reduces Exposure to Hazardous Vapors
• N-95, N-99 or N-100 respirator

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Eye Protection and Goggles

• Working with Aquatic Animals or Primates
• BSL 2, 3 4 Areas
• Performing Invasive Surgery
• Handling Hazardous Samples

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Animal Room Considerations

• NOTE EXTERIOR POSTINGS CAGE CARDS
• WEAR APPROPRIATE PPE
• USE LAMINAR FLOW HOODS WHEN HANDLING ANIMALS
• KEEP NOISE TO A MINIMUM

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Handling Laboratory Animals

• Transporting animals
• Handle in a secure, quiet location
• Do not leave animals unattended!!
• Consider the use of guards or muzzles

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Restraint Techniques

• Manual Restraint
• Chemical Restraint

• Mechanical Restraint

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Laboratory Considerations

• DEDICATED HANDLING AREAS
• POST STANDARD OPERATING PROCEDURES
• SANITIZE AND MONITOR BENCHES
• LABEL SAMPLES WASTE

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Understanding Syringes

• Barrel and Plunger
• Luer Fit and Luer Locking
• Units of Measure
• Drawing-up Compounds

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Guide to Hypodermic Needles

• Gauges (14 31ga)
• Color Coding
• Luer fitting
• Uncapping

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Recapping of Needles

• When is it Appropriate?
• What to do
• What not to do

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Handling Biohazardous Waste

• SHARPS
• Needles, Razors and Lancets
• Pipette Tips and Broken Glass
• Anything that might permeate a bag
• Sharps Container (Radioactive Type)

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Handling Biohazardous Waste

• TISSUES
• Animal Carcasses
• Blood and Serum
• Specimens and Fixatives
• Placed into a Dedicated Disposal Cooler

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Handling Biohazardous Waste

• CONTAMINTED REFUSE
• Exam and Surgical Gloves
• Disposable Materials Contaminated with Bodily
  Fluid or Excreta
• Decontaminate Prior to Disposal?
• Biohazard Bin and Radio-waste Types

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Handling Biohazardous Waste

• REUSABLE MATERIALS
• Surgical Instruments and Workstation
• Decontamination Procedures
• Hypo-chlorites, Quaternary Ammoniums

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Animal Exposures

• Bites, Scratches or
• Needle Sticks
• Insect Vectors
• Apply First Aid and
  Notify Instructor or
  Advisor
• Go to Student Health Center or Emergency Room

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Lab Animal Occupational Health Program

• Participation may be required by the IACUC
• Project health risk categories 1, 2 and 3
• 2-sided form to be completed (UAC office)
• Clearance by SHS physician
• Informative resources

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Field Studies
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Field Considerations

• BE PREPARED
• FOLLOW SOPS
• READ POSTINGS
• REPORT BITES EXPOSURES
• CARRY PERMITS CELL PHONE

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Field Preparedness
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Read Postings
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Project-Related Risks
Inherent Risks
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Unanticipated Risks
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University Animal CareDuncan Hall room 244

• Larry Young, RVT, LATg
• Operations Manager
• 924-4929
• Devon Miracle, RVT
• Veterinary Nurse
• 924-6525