DNA

1
Transcription

• ribosomal RNA
• transfer RNA
• messenger RNA

DNA
kProcaryote mRNA
kEucaryote pre- mRNA
2
pre - mRNA

• ??? intron ??? ????????????? exon ????????
• ???? 7-methyl guanosine ??????? 5
  ????????5 caps

• intron ?????????????????????????
  ????????????????
• exon ???????????????????????????????????

3
pre - mRNA

• ???? adenylate mucleotides ?????? 200 ???
  ??????? 3 ???????? poly A tail
• ???????????????????????

4
mRNA
5 G-ppp 5 cap
AAAAAAAAA-OH 3
Poly A tail
CYTOPLASM
5
???????????????? (Translation)
??????????????????? ?????????????????????????????
?????? ???????????????????????????????????????
???????????????????????????
6
Translation

• mRNA
• tRNA
• Ribosome
• Genetic code or Codon

7
(No Transcript)
8
amino acid enzyme
tRNA
Aminoacyl tRNA
9
Ribosome
Procaryote
Eucaryote
subunit
subunit
Ribosome
10
Genetic code

• ???? triplet code ?????? mRNA ????????????????????
  ??
• AUG ????????????????? (initiation codon) ??????
  methionine ???? N-formylmethionine
• UAA, UAG ??? UGA ???????????????? (terminator
  codon)

11
Genetic code

• ???????????? ??????????????????????????
• ??????????????????????????????????????
• ????????? 43 64 ?????

12
2
1
3
13
2
3
1
14
mRNA
AUG
Ribosome small subunit
Ribosome large subunit
GUC
15
fmet-leu
fmet - leu - trp
16
Releasing factor
UAG (stop codom)
Polypeptide
17
(No Transcript)