Using Your Staining Antibodies with BD CompBeads

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Using Your Staining Antibodies with BD CompBeads
Verify Ab has Kappa light chain
Match Ab to species
Mouse - use BD552843
Rat - use BD552844
Rat/Hamster - use BD552845
Single stain beads as if 1x106 cells
Use amount to match or exceed brightness of
stained cells
Run as compensation sample
Use validated instrument settings
Fit beads on scatter plot
Gate on singlet beads
Acquire sufficient events
Flow Core 15,000 all
M. Roederer 50,000 gated
Calculate compensation
060320 / EVC-FCC / Suzanne Mertens /
saguila_at_emory.edu