Vesicular Traffic II

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Vesicular Traffic II
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Endocytic and secretory pathways
red secretory green endocytic blue recycling
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Different coats are used for different transport
steps in the cell
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Assembly and disassembly of clathrin coat
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Dynamin pinches clathrin coated vesicles from the
membrane
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Electron micrograph from Drosophila mutant
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Resting Chemical Synapse
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Drosophila mutant can not recycle synaptic
vesicles
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SNARE proteins guide vesicular transport
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Active Chemical Synapse
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Interaction of SNARE proteins while docking
synaptic vesicle at nerve terminal
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Model for membrane fusion. Tight SNARE pairing
forces water molecules from area between lipid
bilayers
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Entry of HIV into lymphocytes
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Recruitment of cargo molecules into ER transport
vesicles
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Vesicular tubular clusters move along
microtubules to carry proteins from ER to Golgi
apparatus
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Vesicular tubular clusters
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Microtubules
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Microtubules
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DVD Clip 58
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3-dimensional model of the Golgi Apparatus
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Golgi apparatus in an algal cell
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ER Golgi transitional zone in an animal cell
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Some enzymes are enriched in cis or trans
compartments of the Golgi
substrates specific for certain enzymes such as
acid phosphatase
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Functional Compartmentalization of the Golgi
Apparatus
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Golgi apparatus stained with GFP
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Golgi apparatus can be polarly distributedIn
this fibroblast Golgi is facing the direction in
which the cell is crawling
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Drawing of goblet cell in the intestinal
epithelium
Secretes polysaccharide rich mucus into the small
intestine Golgi is highly polarized to
facilitate release of mucus through exocytosis at
the apical domain
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N-linked glycosylation because sugar is added to
N of asparagine.
original precursor oligosaccharide added to most
proteins in the ER
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Oligosaccharide chains are processed in the Golgi
complex
common core
high-mannose
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Vesicular transport model
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Cisternal Maturation Model
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Lysosome interior is different from cytosol
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Detection of acid phosphatase in lysosomesSmall
spheres may be vesicles delivering the enzyme
from the Golgi Apparatus