PLASMA MEMBRANE AND ITS SURFACE STRUCTURES

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PLASMA MEMBRANE AND ITS SURFACE STRUCTURES
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??????

• ??? ???????
• ????
• ?????
• ??? ?????
• ???????????
• ???????????
• ????????
• ??? ???????
• ??????
• ?????
• ?????????

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• ??(plasma membrane)??????
• ????????????
• ???(biomembrane)?????????
• ????????,???????????
• ?????????????????
• ?????????????????????

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??? ???????

• ???????????,???????,??????????
• ?????????,??????????????
• ??????????????????

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????

• ??????????????????
• (?)??
• ?????50???????
• ????????????(????)?
• ????????,16,18?20?????
• ?????????(???)?

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Phospholipids
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1?????

• ??????,???
• ?????phosphatidylcholine,PC(???)
• ??????phosphatidylserine,PS
• ??????phosphatidylethanolamine,PE(???)
• ?????phosphatidylinositol,PI
• ??????Diphosphatidylglycerol, DPG(???)

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Phospholipids in Plasma Membrane
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Diphosphatidylglycerol
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2????

• ???(sphingomyelin,SM)????(sphingosine)????????????
  ????,????????????

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(?)??

• ??5??,????????,??5-10?
• ????,???????,???????????????????
• ????????,???????????????????

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glycolipids
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Glycolipids
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(?)???

• ????????,??????1/3,???????????
• ??????????,?????,????????????

• ??????????,????????????????????

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(?)???(liposome)

• ???????????????
• ??????????,??251000nm?
• ????????
• ???
• ?????
• ????????

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(No Transcript)
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?????

• ???????????30?
• ?????????????
• ????(integral protein)
• ????(peripheral protein)
• ?????(lipid-anchored protein)?

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• ?????????(transmembrane proteins),???????????1????
  ??a??????????,????????????????

??????SDS
???????Triton-X100
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• ???????????????????????????,??????????????????????
  ???
• ?????????????,?????????,???????????

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• ?????????
• ??????(GPI)??????????????,????C????,??????????????
  ??????????????PrPC???????
• ????????????????????????

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?,? integral protein ?,? lipid-anchored protein
?,? peripheral protein
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??? ?????

• ???????????

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1. E. Overton 1895 ????????????????
2. E. Gorter ? 1925 ??????????????

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• 3?J. Danielli H. Davson 1935 ???????????-???????
  ??,???????(???-??-???)?

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• 4?JD. Robertson 1957????????????????7.5nm?

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5?Singer ?Nicolson 1972 ????????????????,????????
??
Fluid-mosaic model
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???????????

1. ??????????????????????
2. ????????????,???????????????
3. ???????????,??????,????????,???????????

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(?)??????

• ??????????????
• 1????????
• ?????????????????
• ?????????????????????

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2???????????

1. ???
2. ????????
3. ???????
4. ???/???
5. ?????????????????

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3?????????

• ????????????????
• ?????????????????
• 4??????????
• ??????????????,??????????????,?????????,?????????

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???????????????
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(?)??????

• ?????????????,????????
• ??????????,??????ES,?????EF????????PS,?????PF,
  ????????

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?????????????
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• 1.????????????????????????,?PC?SM????????,PE?PS?
  ????????????????????,80?PC??,PE?PS????20?10????
  ?
• 2.??????????????????????????
• 3.?????????????C???????M??

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(?)??lipid raft

• ????????????????
• ?70nm??,???????,?????????
• ???????????,???????
• ????????????????????,???????(DRMs)?
• ????????????,?????????????????????

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Mosaic domain model of the plasma membrane
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Mechanisms of raft clustering. (a) Rafts (red)
are small at the plasma membrane, containing only
a subset of proteins. (b) Raft size is increased
by clustering, leading to a new mixture of
molecules. This clustering can be triggered in
different way.
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????????

1. ??
2. ??
3. ??
4. ???????
5. ??????
6. ????????????

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??? ???????

• ??????????????????????????

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??????

• ?????????????,????????????,?????????1020nm???,???
  ????
• ????????????????????
• ???????????AB0?????????,????????,????????A????????
  N-?????B??????O???????????

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(No Transcript)
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Simplified diagram of the cell coat (glycocalyx)
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?????

• ????????????????????0.2µm??????
• ????????????
• ???????,?????????????,???????????,????(ghost)?

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• ?SDS-???????????,???????
• ??????????a??ß???????,?????????????????
• ???(ankyrin)???????3????????,??????????????
• ?????????,????Cl-,?HCO 3 -??????????,??????12??
• ????????????,??????,?MN????,??4.1?????

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• ?????????
• ??????????????????(?1315??)??,???????????????????
  ????
• ???4.1??????????
• ???????3?????
• ????????????????????,??????????????????????????

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(No Transcript)
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(No Transcript)
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?????????

• ?????????????,????,??????????

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(?)???microvilli

• ????????????,????0.1µm ?
• ???????????????????????????(villin)?????(fimbrin)?
  ???????
• ???????myosin I ???????????????
• ???????????,??????????????????????,?????????30??

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(No Transcript)
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????????????????? M????,MV????
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(?)??(ruffle) ?????????,?????(lamellipodia
)????????????????????

• SEM image of alveolar (Lung) macrophage attacking
  E. coli. (Show ruffle on the cell surface)

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(?)?? ??(infolding)???????????????,???????????????
?????
Infolding
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• (?) ?????
• ??????????,????????????,??????????????????

cilia flagella
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Human sperm
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THE END