RNA????? - PowerPoint PPT Presentation

1 / 93
About This Presentation
Title:

RNA?????

Description:

Title: Author: zy Last modified by: zhang Created Date: 7/24/1998 6:49:20 AM Document presentation format: Other titles – PowerPoint PPT presentation

Number of Views:49
Avg rating:3.0/5.0
Slides: 94
Provided by: zy18
Category:
Tags: rna | snrna

less

Transcript and Presenter's Notes

Title: RNA?????


1
? ? ? ?
RNA????? (??) RNA Biosynthesis, Transcription
2
?? (transcription)
  • ????DNA?????RNA??? ? ????DNA????????RNA??????

??
3
?????????
??? ???
??
?? ?DNA??? ????
??? ?4?NTP dNTP
NTP ???? AT
AU,TA ????5?3 ??DNA????
DDDP DDRP ???????? ??????
mRNA?
tRNA?rRNA
4
???????
?? NTP (ATP, UTP, GTP, CTP) ?? DNA ?
RNA???(RNA polymerase, RNA-pol) ???????
5
???
???? Templates and Enzymes
6
??????
  • ????strucural gene
  • ????mRNA????????? ????
  • ???template strand
  • ????????RNA?????????????Watson??
  • ???coding strand
  • ???????????????????Crick??

7
?? ??????????
  • 5 GCATTAGCTAGCTACTAGC 3 DNA
  • 3 cgtaatcgatcgatgatcg 5 ??
  • ??
  • 5 GCAUUAGCUAGCUACUAGC 3 mRNA
  • ??
  • N Ala?Leu?Ala?Ser?Try C ??

8
???????
  • ?????asymmetric transcription
  • DNA?????????,?????,???????
  • ???????????

9
????
5? 3?
3? 5?
???
???
???
???
10
?? RNA???
  • ?????DNA?RNA???
  • DNA dependent RNA polymerase
  • DDRP

11
?????RNA???
  • ?????RNA????2???
  • ???2?????????????
  • ??????????
  • ? ?2????
  • ?????????(?????)???????

12
??? (core enzyme)
?? (holoenzyme)
13
RNA??????????????
14
?????RNA???
?
15
?????RNA???
RNA???II?????????????RNA???
16
???????????
???????????????????,?????(operon),?????????????(up
stream)??????
RNA???????DNA???,?????(promoter)?
17
???????????
  • ????????
  • ?????????
  • -35bp?????5-TTGACA-
  • -10bp?Pribnow? 5-TATAATPu
  • ?????????(?-30?Hogness?TATA?)?

18
RNA??????
? ?
19
????
-35 ?
-10 ?
T T G A C A A A C T G T
T A T A A T Pu A T A T T A Py
RNA-pol???? (recognition site)
(Pribnow box)
???????????
20
????
-GCGC---CAAT---TATA
???????????
21
???
???? The Process of Transcription
22
???????????
(?)????
  • ???????????
  • RNA?????????????????????
  • DNA????,???????????????

23
??????
1. RNA?????(?2????)?????
2. DNA????
3. ?RNA???????????????,?????????
5?-pppG -OH NTP ? 5?-pppGpN - OH 3? ppi
???????
RNApol (?2????) - DNA - pppGpN- OH 3?
24
????????
  • ??
  • ??????
  • ???????GTP?ATP
  • ???????(RNA-?????-DNA-pppGpN-OH)
  • ?????????, ?????????????????RNA????DNA??????

25
????(transcription bubble)
RNA-pol (???) DNA RNA
26
(?)????
1. ?????,RNApol????????,???????,??DNA????
2. ???????,NTP????,RNA??????
(NMP) n NTP ?? (NMP) n1 PPi
27
?????
  • ???????5?3???????
  • (NMP)nNTP?(NMP)n1PPi
  • ?GC?AU?TA?????
  • ???????DNA???????
  • ???RNA????????,????????,?????,??????????

28
(No Transcript)
29
?????
30
?????
31
DNA
RNA
5?
3?
RNA???
???
???????????????
32
(?)?????
  • ?????RNA??????????????,RNA?????????????
  • ????Rho???????????Rho????????

33
1???Rho???????
  • Rho???????RNA-DNA?????????,?????????????????????

34
1. ?? Rho???????
A T P
35
2. ??? Rho???????
DNA????????,?????????,???RNA?,RNA???????????????
36
2????Rho???????
37
DNA
5?TTGCAGCCTGACAAATCAGGCTGATGGCTGGTGACTTTTTAGTCACCA
GCCTTTTT... 3?
5UUGCAGCCUGACAAAUCAGGCUGAUGGCUGGUGACUUUUUAGUCACCA
GCCUUUUU... 3
5UUGCAGCCUGACAAAUCAGGCUGAUGGCUGGUGACUUUUUAGUCACCA
GCCUUUUU... 3
5UUGCAGCCUGACAAAUCAGGCUGAUGGCUGGUGACUUUUUAGUCACCA
GCCUUUUU... 3
RNA
??(stem-loop)/??(hairpin)??
38
????????????
  • ?RNA?????,????
  • ??????????,RNA?????

39
???Rho?????????
RNA????-???,?? ?????? 1.???????RNA??? ???,????????
? 2.DNA?RNA??????? ????,???????? ?,?????????? ?? ?
??????U,???? ?RNA????????? ?????
40
???????????
(?)????
?????????????????????,?????,RNA-pol???????,???????
??????
41
1. ??????????
???
??????(cis-acting element)
????
AATAAA
???
?????
?????
?????
???
???
TATA?
OCT-1
CAAT?
GC?
OCT-1ATTTGCAT???
42
2. ????
?????????????????DNA????,???????,?????????(trans-a
cting factors)?
???????,???????RNA????,???????(transcriptional
factors, TF)?
43
??RNA-pol????TF?
44
3. ???????? (pre-initiation complex, PIC)
????RNA-pol??DNA??????,????????????
45
?RNA-Pol ??????PIC
TAF
TBP
?B
TATA
?A
TF?D-?A-?B-DNA???
?H
?E
?H
?E
TAF
TBP
TF?F
?B
TATA
?A
PIC????,TF?H?CTD???
46
4. ????(piecing theory)
?????????????3?5????????????????,?????,????????,??
RNA??????????????????????
47
(?)????
???????????????????,???????,?????????????
RNA-pol???????????
???????????????????????
48
???????????
???
RNA-Pol
????
RNA-Pol
RNA-Pol
49
(?)????
???????????
AAAAAAA 3? mRNA
5? ------AAUAAA--
3???
5?------AAUAAA-
???
-GUGUGUG
RNA-pol
5?
3?
AATAAA GTGTGTG
5?
3?
????????
50
???????????
  • ????mRNA???????????,??????????
  • ?????????3???,????????AATAAA,?????????GT??,??????
    ?????????

51
??????????
  • ??DNA???
  • ????????,?5?3???????????????
  • ?????????
  • ?????DNA????
  • ???????????

52
????????
53
???
?????????? Post-transcriptional Modification
54
??????
  • ?????RNA,?????????
  • ?????????????,??????????????????,????????

55
?????????
2. ??(cleavage)
1. ??(splicing)
3. ??(modification)
4. ??(addition)
56
??????mRNA??????
(?)??????
  • 5???? ????(m7GpppGp )
  • 3???????????(poly A tail)

57
????
58
???????
5? pppGp
59
????mRNA??????
  • 5?3?????
  • ??

60
5??
  • ???????????pppG???5-ppG?5-pG
  • ?????????????????
  • ??????????
  • ?????hnRNA?,???????????,???????

61
3?????????
  • ????mRNA?poly A????????DNA????
  • ??poly A??,?????????3??????????,????polyA?
  • 3??????????,?????????
  • Poly A??????,???mRNA?????????,????mRNA???????

62
3??????
63
(?)mRNA???
1. hnRNA ? snRNA
  • ?????mRNA?????RNA (hetero-nuclear RNA, hnRNA)
  • snRNA (small nuclear RNA)

64
????(splite gene)
????????,?????????????????????????,??????????,????
??????????????,???????????
??? A?B?C?D
65
2. ???(exon)????(intron)
  • ???
  • ????????????????,??????RNA??????
  • ???
  • ?????????????????????????

66
?????????????????
???????
hnRNA
?????
hnRNA??
???mRNA
67
mRNA
DNA
???????mRNA?DNA?????
68
????mRNA???
  • DNA???hnRNA??????,????mRNA?hnRNA?DNA??????????
  • ??????????????

69
????
  • ?????????,?????????????????,????????,?????????????
    ???
  • ?????????????????????
  • ??????????????
  • ????????????????,???????

70
??????
  • ???,???????rRNA??
  • ???,????mRNA
  • ??????????,?SnRNA????????????????????
  • tRNA???

71
3. ??????
?????????????,????????4??
I?????????????????????? rRNA?? II???????????,??
???mRNA III?????????????,???mRNA????????
IV?tRNA???????????????,???????ATP?
72
4. mRNA???
??hnRNA?????,???????
  • snRNP?hnRNA???????

?
73
?
?
74
  • ???????????
  • (twice transesterification)

pG-OH (ppG-OH, pppG-OH)
75
5. mRNA???(mRNA editing)
  • RNA??????,??????????????,?????????,?????????(dif
    ferential RNA processing)?

76
??????
  • ?????????????
  • ????

77
tRNA??????
  • ??????5??16??????????14????????

78
??tRNA??????
tRNA??
79
(No Transcript)
80
tRNA??????????
ATP
ADP
81
????
82
???????
  • ???
  • ??
  • ????????
  • ????
  • 3???CCA-OH

83
??rRNA??????
84
rRNA??????
  • ??????????????????????????????
  • ?????rRNA?????????
  • 45s????????rRNA???,??????????????18S
    rRNA,????5.8S?28S?rRNA?

85
???rRNA???
86
???rRNA???????????
???rRNA????????
5-??????
87
??(ribozyme)
  • ??RNA????????,???RNA????????,?????
  • ????????????

88
  • ?rRNA?,tRNA?mRNA??????????????
  • ???????????????
  • (hammerhead structure)
  • ???60??????
  • ?????????????????
  • ???????????????

????
89
???????
  • ??????????
  • ?????????
  • ?????????????

90
  • ???????
  • ?????,???????????
  • ??????????????
  • ??????????????? ?

91
???????
  • ???????????
  • ???????????
  • X ???????
  • ???????

92
??
93
???RNA?????
Write a Comment
User Comments (0)
About PowerShow.com