Comparable between Rapid One Step

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• Comparable between Rapid One Step
• Immunochromatographic Assay and ELISA
• in the Detection of Prostate Specific Antigen
• in Vaginal Specimens of Raped Women
• Vichan Peonim MD,
• J Med Assoc Thai 2007 90 (12) 2624-9

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???????????? (objective)
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• ???????????????????????????????????????
• - rapid one step test kit
• - ELISA
• ??????????????????????????????????????????????????
  ?????????? ?????????????????????????????????
  ????

4

• ?????????
• ???????????????????????????????????
• ????????????????????????????????????????????????
• ???????????????????????

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????????????????????????????????????????????????

• ??????????????????????? (Vaginal Specimens of
  Raped Women)
• ???????? (sperm) ?????????????????
• ???????? (semen)
• ????????????????????????????? semen

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?????????????

• ???????????????? Acid phosphatase test
• ?????????????????????????????? PSA (prostate
  specific antigen)
• ???????????? PSA ??????? quantitative
  ??????????????????????? ELISA
• Rapid one step test kit (semi-quantitative)
  ???????????????????????

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????????????????????
????? ???????
ELISA -Quantitative -Cut off ??? -??? -??????? -???????????????
Rapid test kit -??? -?????????? -?????????????????? Semi-quantitative
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?????????????
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PSA

• E1 , P30
• ???????? glycoprotein ?????????????? prostatic
• ?????? PSA ?????? chromosome 19
• ??????????? PSA ?????????????????????????????????

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???????????????????
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Spermatozoa

• ????????????? ?????? ?????????????????
• ??????????????????? meiosis

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????????(????) ????????????????????
????????(??????) ????????????????????
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??????????????????
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???????????????? ??? ???????? (spermatozoa)
?????????????????? ??? ?????? (seminal plasma)
semen
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semen

• ?? 1 ????????????????? ???????????????? 20-200
  ???????
• ????????????????????????????????????????? 3-5
  ?????????????????
• Seminal vasicle ?????? 70
• Prostate gland ?????? 13-33

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ELISA

• ??????????????????????????????? solid phase
• ??????????????????????????????????????????????????
  ???????????????
• ???????????????????????????????

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• ?????????????????????????? ELISA ???
• Horseradish peroxidase
• (substate ??????? ??? O-phenylene diamine
  ???????????)
• alkaline phosphatase
• (substrate ?????????? p-nitrophenyl phosphate
  ???????????)
• Cut off 0.5 ng/ml

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Rapid one step test kit (Immunochromatography)

• ?????????????? (solid phase)
• ?????????? nitrocellulose ???? nylon
• ???????????????? ?????????????????????????
  ? ??????????????????????
• ??????????? ???? ????? (colloidal gold)

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Rapid one step test kit (Immunochromatography)

• ???????????? PSA ??? rapid one step test kit
• test (mouse anti-human PSA Mc antibody)
• Antibody colloidal gold conjugate
• ????????????? PSA (specific antibody- PSA-color
  conjugate)
• ???????????????????????????????????? 100 µl ( 3
  ???)
• cut off ??? PSA ??? 4 ng/ml

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Rapid one step test kit (Immunochromatography)
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• ??????????????
• ????????????????????????
• ???????????????????????
• ????????????????????????
• ??????????????
• ???????????????? Acid phosphatase
• ??????????????????????????????????
• ??????? PSA (prostate specific antigen)
• ???????????????????????????????

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???????????? (material-method)
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???????????? (material-method)

• ?????????? ?????????????? ??????????????????
  ???????????????
• ???????????????? 100 ???????? ??????????
• N ????????????????????? 96
  
• ? 0.05 ???? 1-a/2 (alpha error
  two-tailed)
• Z Z0.975 1.96

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???????????? (material-method)

• N 59 ????????????????????? 96
• ????????????????????? 67.5
• ?????????????? ????????? 87 ????????

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???????????? (material-method)
- Cotton swab - ?????????????????????? 0.3 x
0.3 cm
- ??????????????????????????? (DW) 2 ml
??????????? 3 ????
- ????????????? rapid one step test kit ??? ELISA
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???????????? (material-method)

• ???? 2 ???????
• ?????????????????????????????????????????????
  (gold standard)
• ??????????? sensitivity, accuracy,specificity,
  positive predictive value, negative predictive
  value

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?????????? (result)
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Result

TP FP FN TN
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Result

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????????????????? (discussion)
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????????????????? (discussion)

• ?????????????????????????????????????? rapid one
  step test kit ????????????????? seminal fluid
• ??????????????????????????????????????????????????
  ???????????????????????
• sensitivity
• negative predictive value
• ????????????????? rapid kit ?????? ELISA

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???? (conclusion)
35
????

• rapid one step test kit ?????????????? PSA
  ??????????????????????????????????????????????????
  ???????????

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???????????????? ?.????? ?????????
thank you