ELISA!

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Lecture 6

• ELISA!

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E
Protein that facilitates a chemical reaction
L
I
Immune system - antibodies
S
A

• Survey technique
• High throughput
• Overview

ssay
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Antibodies?
Virus
H-bonding signature
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Antibodies?
How can you use antibodies to detect it?
Atrazine
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Antibodies?
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How ELISA works

• Competition for the antibody active sites
  between
• Analyte of interest - Antigen (ie. Atrazine)
• Analyte mimic or antigen mimic

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The Major Players
Compound of interest

Atrazine
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The Major Players
Competitor

E
ELISA
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The Major Players
The Antibody
ELISA
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Detection
via enzyme catalyzed reaction
Blue
Yellow
E
H2O2
Compound that is detected
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Cross-reactivity
Atrazine
Desethyl Atrazine
Simazine
Ametryn
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