HPLC

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• ?????????
• HPLC
• ????????????
• feature of HPLC
• ?????????
• general process and main assembly of HPLC

high performance liquid chromatograph
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feature and instrument of HPLC
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???????? high performance liquid chromatograph
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?????(2)
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?????(3)
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?????(4)
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???????????? feature of HPLC
?????????? ?????????????????????????
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????????? process and main assembly of HPLC
1.??
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2.????

• (1) ?????
• ??????,??150350105 Pa?
• ??????????????????(lt10µm),???????????,????????,???
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(??)
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(2)??????
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(3) ????
???????????, ???????????????,
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(4) ?????
?????????,??16 mm,??540
cm?????????????????????
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(5) ???????
a. ????? ????,?????????????
?? ???? ????? ????????(1mm
10mm ,?? 8µL) ???????????????
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b. ??????????
?????????? ??????????1024?????
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(No Transcript)
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c. ???????(differential refractive index
detector)
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??????(?????????????)
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d. ?????(fluorescence detector)
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?????
??? ???????????? feature and instrument of
HPLC ??? ??????????? basic principle and main
separating type ??? ??????? stationary phase and
mobile phase ??? ?????????????? factors
influenced separation and choice of operation
condition ??? ????? ion chromatograph ???
??????? supercritical fluid chromatograph
??