RNA????????????

1
RNA????????????

2
??

• ?????????????
• RNA??????????????
• ??????????
• RNA??????????
• ??

3
??

• ?????????????
• RNA??????????????
• ??????????
• RNA??????????
• ??

4
?????????

• ??????????????
• ??,??
• ?????????????-?????????
• ???
• ???,Elisa, ????,etc
• ????????-????????
• ?????

5
?????????

• ????????????????,?????????????????????????????

?? ????????? ??????????
6
??????

• ????
• DNA (??????)
• ??????
• RNA (????)
• ??????

7
DNA vs. RNA(?)

• DNA??????,RNA?????
• DNA???,RNA????

8
DNA vs. RNA(?)

• ??RNA?????????

??rRNA???,??????????????????
9
???RNA????

• NASBA(??)
• TMA(??)
• SAT(??)

10
SAT????
11
SAT?PCR??????
RNA
DNA
12
SAT????(??????)
13
??

• ?????????????
• RNA??????????????
• ??????????
• RNA??????????
• ??

14
DNA?????????

• ???????,???????
• ????????? (????)
• ?????????????
• ?????
• ??????

15
SAT?????16SrRNA?????
RNA?????????,???16s rRNA??10000-20000?
16
SAT?????16SrRNA??????

• - 16SrRNA???????
• ????MG?16SrRNA??,PCR???????100,?????????

Jensen, 1994
17
16SrRNA????,?????,?????
?????????1500bp, ??50??????
18
RNA??(TMA?SAT)?????????????
19
?DNA??,RNA????????????

• DNA????,???,??????????????,????????2-3???
• ltlt????????gtgt???,??PCR??????,???????3-4??
• RNA????,???,??????2-3??????
• ???????,?? ,????????????
• ?????,???????????
• ???????RNA????
• ??????RNA???

20
???????

• ?????????,?????,?????

????M. Chernesky, D. Jang, K. Luinstra, et al.
2006. High Analytical Sensitivity and Low Rates
of Inhibition May Contribute to Detection of
Chlamydia trachomatis in Significantly More Women
by the APTIMA COMBO 2 Assay. J. Clin.
Microbiology 44 (2) 400-405.
21
SAT???????????????
SAT???? SAT??? ???????
????16SrRNA ????,????? ???? ????????? ?????? ???????? ????????????
????RNA ?????,????????? ?????????
??????? ???????(?????) ???? ?????????? ????????????
????42??? ????????? ?????? ????????? ????????? ????????????
22
??

• ?????????????
• RNA??????????????
• ??????????
• RNA??????????
• ??

23
????????????RNA?????(?)

• ???RNA????????????

??FDA?CE ???? (TMA)
??SFDA ?? ?? (SAT) ?????(CT)???????(RNA????) ??
???(NG)???????(RNA????) ?????(UU)???????(RNA????)
?????(MG) ??????? (RNA????) ??????(TB)???????(RNA
????) ????71?(EV71)???????(RNA????) ?????A16?(CA16
)???????(RNA????) ????(EV)??????????(RNA????) ????
?(MP)???????(RNA????)
24
????????????RNA?????(?)

• ???RNA????????

25
????????????RNA?????(?)

• RNA ??CT/NG???(TMA)???????????

• TMA(APTIMA Combo 2) ???????CT/NG???
• ???, 85???????TMA??CT/NG??
• ??????????TMA,?????????CT/NG????
• ????CT/NG????????????TMA( APTIMA Combo 2 )?????

?????Internal training, Gen-probe
26
????????????RNA?????(?)

• RNA ??TB???(TMA)????????

• 2009??CDC??NAAT??TB???????????
  ????TB?????,???????,????TMA?????????
• (TMA??FDA ????????????,PCR???FDA ?????????)

Updated Guidelines for the Use of Nucleic Acid
Ampflication Test in the Diagnosis of
Tuberculosis (2009) United States
27
??

• ?????????????
• RNA??????????????
• ??????????
• SAT???????????????
• ??

28
?????? MG????

• ????
• ????16SrRNA,????,????
• ????
• ??????????????
• ?????????
• ????
• ?????RNA
• ????RNA?????
• ???2-3?,RNA???????
• ????2014?4?

29
SAT??MG????????????

• ???????????????? SAT?????????????

???? ???? ??
?? ?? ??
???????SAT?? ?? 43 0 43
???????SAT?? ?? 5 249 254
?? ?? 48 249 297
?????43/(435)10089.58 ?????
297/(2970)100100 ???(43249)/29710098.32
30
SFDA????????????????

• ??????????????????????????????
• ????SAT??????????

???????MG-SAT?? ???????MG-SAT?? ??
?? ?? ??
???????MG-SAT?? ?? 39 4 43
???????MG-SAT?? ?? 5 249 254
?? ?? 44 253 297
??????????????(39249)/29710096.97
31
SAT???????????

???? ???? ??
??? ? ????????
??? ? ????????,?????
??? ? ??????
PCR ? ?????,??DNA????3???
SAT ? ?????,??
RNA????
????????
????????
32
SATMG?????????
?? ??
??????????? 1???????(????????????) 2??????,????(????) 3????????? 4?????,?????????
???16SrRNA ?????????,?????????,??????????,??????????????
?????,RNA???? ??????? 1????????? 2?????????????,???????? 3?????????? 4???????????
2.5?????? ???????????,????????,??????
33
??

• ?????????????????????????????
• RNA????,??SAT,??????????????????
• ????,????????,RNA?????????????????????

34

• ??!