Methods for studying Adipocyte Lipolysis

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Methods for studying Adipocyte Lipolysis

• Whole-Body Lipolysis
• Ra of glycerol in the bloodstream
• Measuring the dilution of tracer-labeled glycerol
  in plasma (isotopic dilution)
• Has some possible inconsistencies
• Regional Lipolysis
• Lipolytic activity can be assessed in individual
  tissues using arteriovenous methods
• Evaluation of the balance between delivery and
  release of glycerol across adipose tissue bed.
• Done by measuring arterial venous glycerol
  concentrations and adipose tissue blood flow
  (ATFB)
• Methods were arterial and venous catheterization
  and microdialysis probes.

Schematic diagram of a microdialysis probe and
the method used to collect samples
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More Measurement Techniques

• Measurements of hormones, metabolite
  concentrations and blood lactate concentrations
  were measured by Goto via different methods
• Epi Ne measured with high performance liquid
  chromatorograph kits
• Serum GH measured with radioimmunossay kits
• Serum Insulin measured with Enzymic immunoassay
• Serum FFA Ketone Body measured with Enzymatic
  methods
• Serum Glycerol measured with Enzymatic
  colorimetric
• Lactate measured by pricking a fingertip for a
  blood sample