Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome ?????? ????????? ?? ??? ??? ?????? ??-??? ????????? ???? APC - PowerPoint PPT Presentation

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Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome ?????? ????????? ?? ??? ??? ?????? ??-??? ????????? ???? APC

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Title: Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome ?????? ????????? ?? ??? ??? ?????? ??-??? ????????? ???? APC


1
Orientation of asymmetric stem cell division by
the APC tumor suppressor and centrosome??????
????????? ?? ??? ??? ?????? ??-??? ?????????
????APC
  • Yamashita, Y.M., Jones, D.L. and Fuller, M.T.
    (2003). Science 301, 1547-1550

????? ??? ????, ?????? ?????? ????? ?????
????????, ?????? ?????? ??????, ???? ????? ????
2
????? ???????? ?? ??? ???
???? ????? "????? ???? ??? ??????-????? ???????
?? ???? ?????" ??? ??? ???, ?????? ????? ????
"??? ???????? ???????-???? ??????"
3
???? ????? ?? ???? ??? ??? ?????? ?? ??? ????
???? ????????
4
??? ???? ?????? ?????? ?????? ?? ?????????
  • ??? ???? ?????? ???? ?? ?-9 ???? ???? ??? ?? ????
    ??????? ??? ???? ??????? (hub)
  • ??? ???? ?????? ????? ???? ????? ?-hub ?????? ???
    ???? ?? ???? ?? ??? ?????? ?? ????? ???? ???
  • ????? ???????? ??-hub ?????? ???? ????? ??????
    ??????? ???? ?????? ????????? ???? ???

5
. ?-100 ???? ???? ????A-C ??????? ??????-????
?????? ?????? ????? (?? 2 ???? ??? ???? ???? ??
?????? ??????)
. D-F ????????? ???? ????????? ???? ?????. ????
?????? ??? ??????????? ???? ??? ?????.
????
GFP-a-Tubulin
????
Ab to g-Tubulin
????
Ab to a-spectrin
6
?????? ????? 1
  • ??? ???? ???????? ???? ?????? ???????? ?? ??? ???
    ???? ???? ?-hub ???? ???? ????? ??????
  • ????????? ?? ???? ?????? ?????? ??? ????
    ??????????, ???? ????????? ???? ????? ???? ????
    ??? ????? ???? ?? ?-hub
  • ???? ????? ??????????? ??? ??????????? ???? ????
    ???? ?? ???, ?????? ???? ?????? ?? ???
    ??????????? ??????

7
(No Transcript)
8
??? ??? ?????? ?? ?????? cnn - ???? ?????
?? ????? ????? - ???? ??? ???? ???? ???? - ??? ??
?????? ????? -??? ??? ?????? ??????? ??????
9
  • ?????? (?????? 1 ?-2)
  • 1. ?? ???????? ????? ?????? ???? ???? ????????
    ???? ?? ?????????
  • 2. ??? ????????? ????? ?????? ????? ??????
    ?????? ?? ??? ???? ???? ?? ?????? cnn
  • ?????
  • ???? ????? ???? ?????????? ???? ???????? ????
    ???? ????????

10
??????, ??????? ???.??.??. ?????? ?????? ???? ???
??? ???? ????? ????? ????
????? ????? ???.??.??. ??? ???????? ????? ??????
11
?????? ????????? ?? ??? ??? ???? ??????????
?????? ??-???
  • ?????? ???????? ????? ?? ?????? ??? ???? ????????
    ?? ????? ?? ?????
  • ?????? ????? ??????? ?? ????????? ??????? ?????
    ???? ??? ???? ??????? ????? ??? ????? ???? ????
    ???????? ?????

12
????? ??????
  • ?? ????? ??? ??? ??????????? ????? ???? ?????
    ????? ?????? ???????
  • ?? ???? ??? ?????? ??????? ?????? ??? ??? ????
    ????? ????? ??????? ??????? ??? ???? ?hub-?

13
??? ?????? (????? ??????)
  • Armadilodrosophila b-catenin
  • ????? ???-??? ????? ??????? ????????? ???-?????
    ?????
  • cnncentrosomine ????? ????? ?????????
  • DE-CadherinDrsophila Epithelial cadherin ?????
    ?????? ????? ??? ?????? ????? ??? ???? (cell-cell
    contacts)
  • GCSGermline Stem Cells ??? ??? ?????? ??????.
    ??? ?? ?-9 ??? ??? ?????? ????? ???? ???? hub
    ??????? ???? ?????. ???? ???????? ??-hub ??????
    ???? ?????
  • Hub ??? ???? ??????? ???? ?????? ?? ???? ?????.
    ???? ??? ???? ??????? ??? ?????? ??-9 ??? ???. ??
    ?????? ?? ????? ???????? ????? ???? ????

14
??? ??? ??????...????????, ??????? ???????? ??
?????
  • ???????? ???? ?????? ??? ?????? ???? ?????
    ?????? ????? ???????. ???? ? MTOCMicruTubule
    Organizing Center
  • ??????? ???? ????????? ????? ??? ????????? ????
    ?????? ??? ????? ??? ????? ????????? ????? ?? ???
  • ??????? ????? ????? ????? ???? ?????? ?????
    ????? ???? ????? ???. ????? ????? ???? ?????? ?-9
    ???? ?? ??????? ?? ???????????? ???????? ????? ??
    ??????? ??????
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