DNA%20

1
DNA Forensic Evidence Lab
2
DNA Forensic Science Lab

• Predictions
• Is DNA found in the food we eat?
• Where exactly do you think DNA is located?
• Can DNA be extracted?
• Why is DNA extraction an important skill for a
  forensic scientist?

3
(No Transcript)
4
How does forensic id work?

• Every species has their own DNA sequence
• Scan 13 different regions (loci) when creating a
  human DNA profile
• Human DNA ProfileDNA Fingerprint

5
Examples of DNA uses in forensic science

• DNA left at a crime scene
• Exonerate persons who are wrongfully accused of
  crimes
• Identify crime and catastrophe victims
• Establish paternity and other family relationships

6
Is dNA effective in id of persons?

• DNA id is effective if used intelligently.
• Example Assume that type O blood is found at
  crime scene. Type O occurs in about 45 of
  Americans. If investigators type only for ABO,
  finding the suspect with type O blood doesnt
  reveal much.
• Its usually a chain of biological and physical
  evidence like hair, footprints that leads to the
  making of the match.

7
What are some of the dna technologies used in
forensics?

• RFLP Restriction Fragment Length Polymorphism
• PCR Analysis Polymerase Chain Reaction
• STR Analysis Short tandem repeat technology
• Mitochondrial DNA Analysis related to the DNA
  loci passed on from the mother
• Y-chromosome analysis passed on loci from the
  father

8
Interesting Uses of DNA in forensic Identification

• Identifying September 11th victims
• Migrational Patterns of Human throughout history
• DNA Banks for Endangered Species
• Poached Animals

9
Materials Function

• Strawberry large genome due to the fact that it
  is an octoploid (8 times the amount of genetic
  info.)
• Soap dissolve the phospholipid bilayers of the
  membrane and organelles (plant cell)
• Salt break up the protein chains that bind the
  nucleic acids
• Ethanol is kept cold on ice because the DNA is
  insoluble making it easy to separate from the
  beaker.

10
conclusion

• Online is posted the conclusion worksheet.
  Answer all questions on your own sheet of paper.
  Attach your answers to the rest of your lab
  report.
• Lab report due Wednesday.