Semen collection and evaluation - PowerPoint PPT Presentation

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Semen collection and evaluation

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Title: Slide 1 Author: Benq Last modified by: Admin Created Date: 11/19/1996 11:22:43 AM Document presentation format: – PowerPoint PPT presentation

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Title: Semen collection and evaluation


1
Semen collection and evaluation
?????????????????? ???? 117331 Physiology of
domestic animals ??????? ???????? ??????????
2
Semen collection ??????????????????
????????????????????????????????
?????????????????????????????????????????
?????????????????? ???????????????????????????????
????????????????? ?????????????????????????
??????????? ??????? ?????????? ?????????
?????????????????????? ???????? ??????????????
3
  • ?????????????? ??????? ??????????
  • - ??????? ????? ??????????????? ??????
  • - ?????? ???????????? ????????????????
  • - ??????????? ?????????? (collector) ???????????
  • - ?????? ????????????? ?????????????????

4
2. ??????????????????????? ??????????????????????
?????????????????????? ???????????????????????????
2.1 ??????????????? ??????? ????????????? 2.2
??????????????????? (artificial vagina, AV)
????????????????? ?????? ???? ?????? ?? ??? ???
?????? 2.3 ????????????????????????????
??????????????????
5
2. ??????????????????????? (???) 2.4
?????????????? ????????? vesicular glands ???
ampulla ?????????????????????? ????????????? 2.5
????????????????????????? (electroejaculation
method) ???????????????????????? ????
???????????? ????????? ????????????
?????????????????????????????????? 2.6
???????????????????????? (glove hand technique)
??????????
6
????????????????????????????????? ?????? ?? ???
??? ??? ?????????? ???????????????????? (sexual
stimulation) ????????????????????????????????????
??????????????? ??????????????????? ????????
???????????? - ??????????????????????
?????????????????? ???????????????????????????????
? - ?????????????????????????????????????????
7
????????????????????????????????? (???)
???????????????????? ??? ????????? (teaser)
?????????????????????????????????
??????????????????????????????????????????????????
? (fount mount) 2 ??????? 10-15 ????
??????????????????? ??? ????????????? ?????????
??????????? ??????????????????????????????????????
?????? ???????????????????????????????????????????
??????????
8
????????????????????????????????? (???)
???????????????????????????????? ?????? -
???????????????? (outer casing) - ??????????
(inner liner) ????????????? ??????????????????????
???????????? ????????????????????????????? -
??????? (collecting funnel) ??????????????????????
????????????????? ?????????????? 38 56 OC -
????????????????? - ??????????????
9
????????????????????????????????? (???)
??????????????????? ????????????????????????????
???????????????? ?????????????????? AV
????????????????????????????? ???? K-Y jelly
10
????????????????????????????????? (???)
11
  • ????????????????????????????????? (???)
  • ?????????????
  • ?????????????????????????????????????????
  • ?????????????????? ????????????? (sheath)
  • ?? AV ???????????????????????????????
    ??????????????????????????????????????????
  • ?????? ??????????????? AV ??????
  • ????? ???????????????????????? AV
    ????????????????????

12
?????????????????????????????????????
(electroejeculator) ?????????????????????????????
??????????? ???????????????????????????????????
??????????????????? ??????????????????????????? ??
???????? ?????????? - electrode ?? 2 ????
???????????????????? - ???????????????????????
???? 0-30 volt ?????????? 0.5-1.0 ???????
13
?????????????????????????????????????
(electroejeculator)
14
  • ?????????????????????????????????????
    (electroejeculator)
  • ???????
  • ???????????????????????????
  • ???????????????????? electrode
    ??????????????????? ????? accessory gland
    ??????????????????????????????????????????????????
    ???
  • ??????????? volt ??????? ?????????????????
    ?????????????????????????? 0 volt ????????????

15
  • ?????????????????????????????????????
    (electroejeculator)
  • ??????? (???)
  • ???????????????????? 4 ?????? ???????????????????
    ????????? ?????????????????????? ????? 2 5 ????
  • ?????????????????????????????????????????????????
    ????????????
  • ??????????????????? ?????????????????????????????
    ????????
  • - ????????????????????????????????????????????????
    ??????????????????

16
  • ?????????????????? ???????????????????????????????
    ???????????? ????????
  • - ???????????????????????? (cold shock)
  • - ??????
  • ????????????????????? ???? ??????? ???? ???
    ???????????
  • ????????????????????????????????

17
???????????????????????? (semen
evaluation) ??????????????????????????????
????????????????????????? ?????????????????
?????????????????????? ???????????????????????????
????????????? ??????????????????????????????????
???????????????????????????????????????????????
18
???????????????????????? (semen
evaluation) ????????????????????? 1.
???????????? (appearance) ?? ?????
?????????????? ???? ??????????????????????????????
??????????????? ??????????? ??????
????????????????????????????????????????
????????????????????????????????? 2. ???????
(volume) ??????????????? ?????????
?????????????????????????
19
???????????????????????? (semen
evaluation) ????????????????????? 3.
????????????????????? (motility) ???????? 2
??? 3.1 ??????????????????????????????? (wave
motion characteristics) ????????????????????????
(mass movement) ??????????????????????????? ???
40-100 ???? ???????????????????????????????????
????????????????????????? 37oC ???????????????
???????????????????? 0 - 5
20
????? ????? ???????????????????
5 ????? ?????????? ??????????????????? ???? ?????????????????????? ?????????????????? ???? 90
4 ?? ????????????? ??????????????? 5 ????????????????????? 70-85
3 ??????? ??????????????????????????? ??? ??????????????? ????????????????????? 45-65
2 ??? ?????????????????? ??????????????????????? 20-40
1 ?????? ??????????????????????? 10
0 ???????? ??????????????????
21
???????????????????????? (semen
evaluation) ????????????????????? 3.
????????????????????? (motility) ???????? 2
??? 3.2 ?????????????????????????????????????????
??? (proportion of progressive motile
sperm) ???????????????????????? ???????????????
400 ???? ?????? cover slip ?????????????????
?????????????????????????????????????
???????????????????????????????? ???????????????
300 ??? ??????????????
22
???????????????????????? (semen
evaluation) ????????????????????? 4.
??????????????????? (sperm concentration) ???????
????????????????????? / ??. ??????????
???????????????????????????????????? ????
???????????????????? (consistency of semen)
????????????????????????? ????????????????????????
????????? (haematocytometer) ?????? colorimeter
?????????? electronic particle
23
???????????????????????? (semen
evaluation) ????????????????????? 5.
?????????????????? ???????????????????????
???????????????????????????????? ???????????
(differential staining of live and dead sperm)
??????????????? 1 eosin ?????????????????????????
????????????????????????? ????????????????????????
?????????????????????????? ???????????????????????
???????????????????????? ?????? 2 analine blue
???? 5 nigrosin ???????????????????? 2.9 sodium
citrate
24
???????????????????????? (semen
evaluation) ????????????????????? 5.
??????????????????
25
???????????????????????? (semen
evaluation) ????????????????????? 6.
???????????? (sperm cell morphology) ????????????
??????????????????????????? 5 ???????????????????
???????????????? 20-25 ???????????????????????
??????????????????????????????????????????????
????????????????? progressive motility
????????????????????????????????????????
?????????? eosin-nigrosin ??????????????????????
26
6. ???????????? (sperm cell morphology)
27
???????????????????????? (semen
evaluation) ????????????????????? 6.
???????????? (sperm cell morphology)
(???) ???????????????????????????????????? 2
???????? 6.1 primary abnormalities
????????????????????????????????????
?????????????????????? ????????????????? ??????
pyriform or pear-shaped, round, elongated or
slender, microcephalic or small, macrophalic or
giant, double or twin ??? abnormal acrosome
28
6.1 primary abnormalities ????? ?????????????????
Pyriform or pear shape
29
6.1 primary abnormalities ????? ?????????????????
Double head or twin
30
6.1 primary abnormalities ????? ?????????????????
Elongated or slender
31
???????????????????????? (semen
evaluation) ????????????????????? 6.1.2
?????????????? midpiece ?????? ????????? (bent),
???????? (double) ??? (swollen)
???????????????????????????????? (off center
attachment) 6.1.3 ????????????????? ??????
??????? (coiled or curled) ??? 2 ??? (double tail)
32
6.1.2 ?????????????? midpiece Bent
33
6.1.3 ????????????????? Coiled or curled
34
???????????????????????? (semen
evaluation) ????????????????????? 6.
???????????? (sperm cell morphology) (???) 6.2
secondary abnormalities ??????????????????????
seminiferous tubule ????????????????????????
?????? ?????? (detached heads) ??????????????????
(protoplasmic droplet on the neck or tail)
???????? (shochook tail) ?????????? acrosome
(loose cap from the head) ???????????????????????
??????????
35
6.2 secondary abnormalities (???) ??????
??????????????????????????????????????? ??????????
?????? ??????????????????? ???????????????????????
?????????????? ?????????? acrosome
??????????????????????????????????????????????????

36
  • 6.2 secondary abnormalities (???)
  • ?????????? acrosome ???????????????????????
  • Normal apical ridge
  • Damage apical ridge
  • Missing apical ridge
  • Loose acrosomal cap

37
  • 6.2 secondary abnormalities (???)
  • ???????????????????? acrosome ??????????????
  • ????????????? phase-contrast differential
    interference contrast (DIC) ??????????????????????
    ?????????? ?????????? ??????????? acrosome ????
  • ???????????????????????????????????? ????
    eosin-nigrosin, giemsa stain, periodic acid -
    Schiffs reagent, triple stain ???

38
6.2 secondary abnormalities (???) Detached heads
39
6.2 secondary abnormalities (???) Protoplasmic
droplet (proximal droplet)
40
6.2 secondary abnormalities (???) Protoplasmic
droplet (distal droplet)
41
???????????????????????? (semen
evaluation) ??????????????????????????
??????????????????????????????????????????????? ??
?????????????????????????????? - Zona-free
hamster ova test - Hypo-osmotic swelling
test ????????????????? (metabolic reaction
rates) - methylene blue reduction test ?????????
???? ?????????????????, ??????????????????????????
??? ????????????????????????????????
42
???????????
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