Things to Do Today

1
Things to Do Today

• Exam Go over Exam.
• Throat Cultures
• Unknown gm (-) rod ID
• Assign HW Fill in Table 1. Gram ID

2
BAP Hemolytic Reactions
Used with permission
3
BAP Hemolytic Reactions
Alpha hemolysis
Beta hemolysis
gamma
KM
4
Perform throat Swab

• 1 BAP per person
• Swab first Quadrant and then smear swab on slide
  (throat)
• Use loop, flaming in between quadrants, and
  finish the 4 quadrant streak.
• Heat fix the slide

throat
plate
5
Gram (-) Test results
Organism oxidase Urease MR H2S Gas
Glucose Lactose Citrate
Escherichia - -
-
- Pseudomonas
- - - - -
- Morganella
- - -
- - Proteus
-
-
- Enterobacter - -
- -
Salmonella
- - -
-
Providencia -
- -
- Unknown microbe
6
Oxidase Test
Positive
Negative
7
Urease Test
Negative Positive
KM
8
Methyl Red Test
Negative Positive
9
KIA Slants
uninoculated
10
KIA Slants
uninoculated
KM
11
Citrate test
Negative
positive
KM
12
Gram Negative Unknown

1. Obtain unknown and write down number in your
   notes and on ALL media
2. Inoculate the MR and Urease broth tubes
3. Inoculate the Citrate agar by streaking its
   surface
4. Inoculate the KIA by streaking its surface AND
   then stabbing the butt.