Microscopy

1
Microscopy

• Lab 3

2
Microscopy

• Microscopes are used to view things that are too
  small to see without help.
• Dissecting microscope view entire 3-D objects
  under low power.
• Compound light microscope examine very small or
  thinly sliced objects under higher magnification.

3
Microscopy

• Stains that bind to cellular structures are used
  to help increase the contrast and allow you to
  see the structures more clearly.

4
Using the Microscopes

• Always use 2 hands to handle the scopes!
• Be VERY CAREFUL when moving the microscopes.
• Never remove any part of the microscope.

5
Using the Microscopes

• ALWAYS adjust the nosepiece so that the LOWEST
  OBJECTIVE LENS is in place when removing a slide
  and when putting the microscope away!
• NEVER, EVER use the COARSE FOCUS when you are
  working with high power!

6
Using the Microscopes

• Always start with the lowest power lens this
  gives you the widest field of view.
• Find what you are looking for, CENTER and FOCUS
  as well as you can.
• Then, change to the next objective lens.
• Return to low power before removing the slide.

7
Rules

• Lowest power objective lens in position at
  beginning and end.
• Use only lens paper for cleaning lenses.
• Do not tilt the microscope when you are using a
  wet mount.
• Keep stage clean dry.
• DO NOT REMOVE PARTS!!!!
• Turn light all the way down, then turn off before
  unplugging.
• Replace cover when finished.
• Report any problems.

8
Total Magnification

• Compound Light Microscopes have 2 magnifying
  lenses.
• Objective lens 4X, 10X, 20X, 40X
• Ocular lens (in the eyepiece) 10X
• Total magnification multiply the objective lens
  (4, 10, 20, or 40) by the ocular lens (10).

9
Field of View

• Field of view the circular area that is visible
  when you look into the microscope.
• Measure the diameter, estimate lengths of objects
  that take up part of the field of view.
• Depth of field play with the focus while
  viewing the slide with colored threads.