Gel Permeation Chromatography - PowerPoint PPT Presentation

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Gel Permeation Chromatography

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Gel Permeation Chromatography (GPC) 1964 J.C.Moore – PowerPoint PPT presentation

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Title: Gel Permeation Chromatography


1
???????
  • Gel Permeation Chromatography
  • (GPC)

2
  • ????
  • ????
  • ????

3
????
  • 1964?,?J.C.Moore???????
  • ????????????????,????????????????????????????(????
    ???????????????????)

4
????
  • ????
  • ????
  • ????
  • ????

5
  • ????
  • ??????(SEC)
  • (Size Exclusion Chromatography)
  • ????????????????????????,????????????
    ??????(??)???????(??)?????????????,???????????????
    ?,???????????,????????????????????,??????????????
    ??????,?????????????,???????????(??????),?????????
    ??(??????)?

6
  • ????
  • ???????????,??????????????????????
  • ???????????,??????????????,?????,????????

7
????
  • ???????????????????????,?????????
  • ????????????????????????,???????????????,????
    ????????

8
  • ????
  • ??????????????????????????????????????
    ???????,?????????????????????????,??????????????
    ???????,?????GPC????,?????????????????,?lgM?t??,??
    ???????????????,???GPC??????????????????????????
    ??

9
????
  • ????
  • ????????????????????,??????????????????
    ,??GPC???????????????????????????????????????????

10
  • ??????
  • ??GPC??????????????????,?????????????
    ?,??????????,??????????
  • ??????????????

  • ?1M1?2M2

  • k1M1a11k1M2a21
  • ?????lgk1(a11)lgM1lgk2(a21)lgM2
  • ???????????????k?a?,?????????????????
    M1?????????????M2?

11
????
  • GPC????
  • ????(??)??????????????????????????

12
  • ???
  • ????????????????????????????????(??)??????????
    ???????????????????????????????,??????????????????
    ?????0.01mL/min?

13
  • ???
  • GPC???????????????????????????????????
  • ??(????????????,???????????????????)????????(
    ???????,????)???????????(??100?,??????????????)???
    ?(?????????)???????????(?????????)
  • ????????

14
  • ????
  • ???????????????????????????????????????????
    ?????
  • ?????????????????????????????????
  • ????????????????????????????
  • ???????????????????????????????????????????
    ????

15
????
  • ????
  • ?????????

16
  • ???
  • ?????????????????????????,???????????
    ??????????????????????????????????????,???????????
    ????,???????????,?????????????????????????????????
    ???,??????????,???????????????????????????????????
    ????????,????????????????????????????????????????,
    ??????????????????????????

17
????
  • ?????
  • ????????
  • ????????
  • ???????

18
????
  • ?????????????????????????,???????????
  • ??????????????,??????????,????????????????,???????
    ???????????????????

19
????
  • ?GPC????????,?????????????????????????
    ?,?GPC???????????????????,????????????????????

20
????
  • ?????????????????,???????????????,????
    ?????????????????????????????????,??????????????,?
    ??0.2µm???????????????????0.2µm?????????,????????,
    ?????,?????????,???????

21
????
  • ??Wyatt Technology Corp????????????????????(???
    ??)
  • ????
  • Styagel R HMW 6E GPC?(7.8300mm)
  • Wyatt OPTILAB RI???
  • Wyatt??????????(DOWN E)
  • ??????Wyatt Technology(Astra 473)???
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