Microscopes

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MicroscopesLab Techniques
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What is a light microscope?

• Light Microscope a device that uses light to
  produce an enlarged view of an object
• An example of a simple microscope is a
  magnifying glass

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What is a compound microscope?

• Compound Microscope contains 2 or more
  objective lenses.
• One lens (low power) produces an enlarged image
• Another lens (high power) further magnifies the
  image

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Magnification vs. Resolution

• Magnification the enlargement of an image
• Resolution how sharp or in focus an object is

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What is an electron microscope?

• Electron Microscope can magnify images more
  than 250,000 X
• Uses electron beams rather than light

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• A Scanning Electron Microscope

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An ant
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Leaf from a Black Walnut tree
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Pollen
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A moth
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A human skin cell
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A cancer cell
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Centrifugation

• Centrifugation a process by which materials
  suspended in a liquid can be separated from each
  other based on density

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Microdissection

• Microdissection tiny instruments are used to
  perform various operations on living cells

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Tissue Culture

• Tissue Culture the technique of maintaining
  living cells or tissues in a culture medium
  outside the body

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Chromatography

• Chromatography any technique that separates
  different substances based on their chemical and
  physical properties

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Electrophoresis

• Electrophoresis the technique for separating
  substances made up of particles that have an
  electrical charge

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Spectrophotometry

• Spectrophotometry a technique of using light to
  analyze samples