TILLING Database

1
TILLING Database

• Introduction, Current Usage and the Big Picture

2
TILLING Parts

• Sequences (Genes)
• Primer Pairs (several pairs per gene)
• Plates (single, pooled, gel) with Plant DNA
• Gel Runs (one run per primer plate )

3
Functionality

• Standard
• Create Entry
• Edit Entry
• Delete Entry
• Import Entries
• Additional
• Plates Pool Plates
• Plates Generate Gel Plate from pooled Mutation
  Hits
• Primers Generate (CODDLE)
• Gel Runs Set / Edit Mutation Hits
• Gel Runs Import Images
• Sequences Mutation Hit Overview

4
Functionality
5
Workflow

• Enter Sequences
• Enter / Generate Primers
• Import Plates, Pool Plates
• 1st Gel Run on pooled DNA
• Identify Hits
• Generate Gel Plate with single DNA
• 2nd Gel Run
• Identify Single Hits

6
Current Usage

• Plants 36.952 (10.926 TILLING M2)
• Seed Bags 43101
• Sequences 75
• Primer Pairs 154
• Single Plates 67
• Pooled Plates 23
• Gel Plates 153
• Gel Runs 1075 (pooled), 166 (single)
• Mutation Hits 2789 (pooled and single)

7
The Big Picture Mutation Evidence
Plant Seed Database
Line Manager
TILLING DB
Sequencing

• Line attribute in
• Plants / Seed Bags
• Each Line can have
• several Mutations
• in different Plants
• Each Mutation
• might have several Evidences
• Gene Overview

Mapping (in progress)
Complementation Cross (in progress)
8
Plant Seed Database

• Plants (with Seed Bag reference)
• Seed Bags (with parent plant(s) reference)
• Ancestor Tree View
• Progeny Tree View
• Multi Edit (several attributes/entries at once)
• Phenotype Edit (several plants at once)
• Label Printing (Plants / Seed Bags via pdf)

9
ZopRA Framework

• TILLING Database and
• Plant Seed Database are using the
• ZopRA Framework created by ZopRATec
• Contact
• peterseifert_at_gmx.de
• Ik3_at_inf.tu-dresden.de