UniProt: Universal Protein Resource

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UniProt Universal Protein Resource
Central Resource of Protein Sequence and Function

• International Consortium
• PIR at GUMC
• European Bioinformatics Institute
• Swiss Institute of Bioinformatics
• Unifies PIR-PSD, Swiss-Prot, TrEMBL Protein
  Sequence Databases

http//www.uniprot.org
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UniProt Databases

• UniParc Comprehensive Sequence Archive with
  Sequence History
• UniRef Non-redundant Reference Databases for
  Sequence Search
• UniProtKB Knowledgebase with Full
  Classification and Functional Annotation

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UniProt Archive (UniParc)

• An archive for tracking protein sequences
• Comprehensive All published protein sequences
• Non-Redundant Merge identical sequence strings
• Traceable Versioned, with Active or Obsolete
  status tag
• Concise no annotation of function, species,
  tissue, etc.
• 5 million unique entries from
• 13 million source-database entries

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UniProt Reference Clusters (UniRef)

• Non-Redundant Reference Clusters for Sequence
  Searching
• UniRef100 for Comprehensive Sequence Similarity
  Search
• 100 sequence identity from all species, merging
  sub-fragments
• Derived from UniProtKB Splice variants as
  separate entries
• Additional UniParc sources (e.g. Ensembl, IPI,
  EMBL_WGS)

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UniProt Reference Clusters (UniRef)

• UniRef90/50 for Faster Searches using Reduced
  Data Sets
• UniRef90 90 sequence identity (35 reduction
  from UniRef100)
• UniRef50 50 sequence identity (65 reduction)
• Representative Sequence for cluster

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UniProt Knowledgebase (UniProtKB)

• Objective Stable, Comprehensive, Fully
  Classified, Richly and Accurately Annotated
• Describe in a single record all protein products
  derived from a certain gene in a given species
• Information Content
• Isoform Presentation Alternatively Spliced
  Forms, Proteolytic Cleavage, and
  Post-Translational Modification (each with FTid)
• Nomenclature Gene/Protein Names (Nomenclature
  Committees)
• Family Classification and Domain Identification
  InterPro and PIRSF
• Functional Annotation Function, Functional Site,
  Developmental Stage, Catalytic Activity,
  Modification, Regulation, Induction, Pathway,
  Tissue Specificity, Subcellular Location,
  Disease, Process

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UniProtKB Report (I)
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UniProtKB Report (II)
http//www.pir.uniprot.org/cgi-bin/upEntry?idPH4H
_HUMAN