FryeEdidin Experiment - PowerPoint PPT Presentation
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FryeEdidin Experiment
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More highly ordered membrane areas but still dynamic ... Western blot analyses of Arabidopsis. plasma membrane fractions. 1249 = AnnAt2. 1250 = AnnAt1 ... – PowerPoint PPT presentation
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Title: FryeEdidin Experiment
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Frye-Edidin Experiment 1970
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Membrane fluidity Frye-Edidin experiment
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Frye-Edidin experiment
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Frye-Edidin experiment
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Lipid bilayer
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Fluid mosaic model of membrane
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Distinct domains in model membranes
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3-D image of lipid microdomains containing
cholera toxin
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Characteristics of lipid microdomains
- Sub-population of cellular membranes
- Insoluble in non-ionic detergents
- Enriched in cholesterol, sphingolipids,
glycolipids, GPI-anchored proteins and specific
integral membrane proteins - More highly ordered membrane areas but still
dynamic - Suggested to represent signaling centers, sites
of endocytosis and pathogen entry
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Typical lipid raft
Cholesterol
Sphingolipid
Phospholipid
Glycosphingolipid
GPI-anchored protein
Lipid Microdomain
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Fluorescence labeling of cholesterol in membrane
microdomains
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Lipid microdomains potential role in polar
secretion
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Western blot analyses of Arabidopsis plasma
membrane fractions
1249 AnnAt2 1250 AnnAt1 E8 pea p35
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