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Provide a model for human annotation. D-ENCODE 2004. 2. Dual genome ... Old fashioned protein sequencing? Mass spectrometry? What about N-terminal clipping? ...
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and rings each have seven subunits ... are two rings per proteasome complex. ... at the same time, or that an internal loop can be the initial engagement site ...
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validation using small RNA cloning. Machine Learning ... of miRs vs. scrambled counterparts against ENTIRE human RefSeq mRNAs, look for ...
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Prediction of eukaryotic mRNA translational properties. ... Chromatin proteins: Growth factors. Protooncogenes. Histones. Protooncogenes. Ribosomal proteins ...
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McMillan et al 1995 Virology 213; 413. Brand et al 1997 JBC 272;8388 ... Damian Purcell. McGill University. Anne Gatignol. University of Colorado. Jens Lykke-Andersen ...
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1. Capping at the 5'-end. 2. Polyadenylation at the 3'-end ... The cap serves several functions, including protection of the mRNA from ...
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The Fragile X Protein FMRP Associates with BC1 RNA and Regulates the Translation ... Acitivity Dependent Metamorphosis of Spines ...
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Identification and quantitation of mRNAs in a mixed population by generation of ... 1 dna:chromosome. chromosome:NCBIM36:1:4729550:4729591:1 ...
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tegument: jelly-like, withVP16 &Vmw65. regulatory. proteins. Herpesviruses ... Tegument regulatory proteins. Vhs: degrades cell mRNAs, causes host shut off ...
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No direct DNA participation in translation ... Cell-free translation system. In 1961 mRNAs not yet isolated. polynucleotide phosphorylase ...
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Biology 12 Mr. McIntyre * * * * * * * * * * * * * * The consequence of this cleavage is that translation of cellular mRNAs stops But the viral RNA is still ...
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... or the half-life of mRNA. As a rule of thumb, the half-life of bacterial mRNAs is a few minutes, whereas that of eukaryotic mRNAs is usually multiple hours. ...
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RISC recognizes and destroys target mRNAs by cleavage in region homologous to siRNA. ... Possibly orients recognition and cleavage of mRNA substrates. ...
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Microarray analysis shows that some microRNAs. downregulate large numbers of target mRNAs ... in flies, haematopoietic lineage differentiation in mammals, neuronal ...
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only transcribes tRNA genes. each has a specific promoter sequence it ... Alternative mRNAs produced from same gene. when is an intron not an intron...
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Hershey and Chase used two different radioactive isotopes to label the protein and DNA ... mRNAs are the 'blueprint' copies of nuclear genes ...
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Plastid Transcriptional Regulation. Transcriptional regulation is often global or ... Stability of some plastid mRNAs increases during greening (psbA), but most ...
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Table 1 Complexes Involved in RNApII PIC assembly. Protein complex Functions RNApII 12 Subunits; catalyzes transcription of all mRNAs and a subset of noncoding ...
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In some mRNAs, the first ATG is in a favorable context, but is still not used. ... Reticulocytes normally make up only a few % of blood cells ...
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An organized body of persistent data and associated ... honeybee. sea urchin. zebrafish. cow. dog. black poplar. RefSeq Accession Numbers. mRNAs and Proteins ...
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Some other sRNAs are known to repress translation intead of activating ... Binding of TPP represses translation of mRNAs that encode enzymes involved in TPP synthesis ...
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Manually review high quality evidence (human mRNAs) for which no faithful models ... mouse, rat, chicken, frog, fugu, tetraodon, zebrafish, and drosophila genomes. ...
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A nuclease was purified from Drosophila embryos that digests target mRNAs: ... In plants, fungi, C. elegans & Drosophila, a RNA-dependent RNA polymerase (RDR) ...
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How do eukaryotic and prokaryotic mRNAs differ? ... What are the 4 ribosomal binding sites? ... 1) Ribosome: Large subunit and Small subunit (Svedberg Units) ...
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Protects mRNA from ribonucleases: mRNA's without caps rapidly ... MT binds to CE (mammals) or P-CTD (yeast) and to the terminal GpppN (mammals). c, Imp ...
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Mother expresses these genes during oogenesis (mum packs a lunch... Earliest stages of development run on mRNA's and proteins deposited by mum during oogenesis ...
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Scanning ? Some of the proteins that bind the 5' UTRs of mRNAs promote translation ... expressed poorly if plastids don't develop, or are damaged by photo-oxidation. ...
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Information matrix for. the mRNAs. the number of s of treatments j and k hybridized ... biological extraction may lead to some correlation between the resulting log-ratios. ...
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0 3 6 10 15 20 30 40 50 60. Tell me in detail how this experiment ... deleterious effect. Premature Polyadenylation Occurs. Frequently at Many Different Sites ...
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... translation can be modelled using a probability-based model that accounts for ... the relationship between mRNA length and ribosome density based on gains ...
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Chap. 8 Post-transcriptional Gene Control Topics Processing of Eukaryotic Pre-mRNA Regulation of Pre-mRNA Processing Cytoplasmic Mechanisms of Post-transcriptional ...
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Controlling the rate at which the mRNA decays can regulate the levels of ... (dsRNA) introduced into the nematode worm, C. elegans, and is largely conserved ...
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... transcribed by RNA Pol II ... cleavage factor II PAP: Poly(A) polymerase CPSF PAP PABPII- poly(A) ... different from mRNA p p Pol II ctd mRNA SCAFs: ...
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Saccharomyces cerevisiae strain Y262 (MATa ura3-52 his4-939am rpb1-1) ... Determination of mRNA Decay by Transcriptional Shut ... of Poly(A) Shortening Rates ...
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AU-rich element-directed mRNA decay. microRNAs and small interfering RNAs ... First identified in Xenopus cytoplasm. Shows high specificity for poly(A) sequences ...
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The aim of my project is to uncover how eukaryotic cells maintain dormant stages ... mRNA is associated mainly with ribosomes and is capped ...
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Transcription by RNA Pol I an II. Regulation of transcription in eukaryotes: ... Introns (non coding sequences) are cut out from the pre-mRNA. ...
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Double-stranded RNA (dsRNA) induced interference of the Mex-3 mRNA in the nematode C. elegans. ... C. elegans (nematode) Drosophila. Mammalian cells. called ' ...
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Nucleic Acid Chemistry contents contents (1) mRNA --messenger RNA 5 cap:m7GpppNm- 3 Poly A tail An n=20 200 Single chain half life ...
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Regulating gene expression Goal is controlling Proteins How many? Where? How active? 8 levels (two not shown are mRNA localization & prot degradation)
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The chromosomal organization of the genes in each Hox cluster reflects its ... In plant, most miRNAs anneal to their targets with nearly complete ...
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These GEFs rapidly cycle between cytosolic and membrane-bound forms ... One example of interference with the mRNA export pathway is viral-host ...
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Many proteins involved in mRNA turnover localize to punctate structures. Figure 1 (Left) ... with fluorescent epitopes have a punctate distribution in cells ...
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5'- Degradation in Yeast. Following deadenylation, 5' to 3' degradation requires ... RNA undergoes extensive processing incompletely / incorrectly ...
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Electron Micrograph of a DNA-RNA Hybrid. tRNA Transcription & Post Modification ... requires mRNA, ribosomes, tRNA, aminoacyl tRNA synthetases, and initiation, ...
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Subtractive hybridization. Differential display ... Subtractive hybridization. mRNA *cDNA (radiolabeled) mRNA. anneal with mRNA in excess ...
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mRNA decay - regulating gene expression - Wiebke Ginter 06.12.10
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rRNA and tRNA are Cogs in the Machinery. rRNA is a structural part of the ribosome ... on how to put together your 'easy to assemble' bookcase/desk/whatever ...
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LECT 20: PROTEIN SYNTHESIS AND TRANSLATIONAL CONTROL High fidelity of protein synthesis from mRNA is essential. Mechanisms controling translation accuracy include ...
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El splicing de oskar mRNA esta acoplado con su localizaci n citoplasm tica Y14 y Mago se colocalizan con oskar mRNA en el polo posterior del oocito de D. melanogaster.
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Mature. mRNA. 06-06-02. Splicing: Letters. DNA ... Mature mRNA (after splicing) better describes the final protein sequence than DNA ... may not be mature mRNA ...
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DNA's information is copied into messanger RNA (mRNA) molecule ... motif called the TATA BOX ... TFIID-protein binds to TATA BOX. Directs the binding of ...
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Macho-1 mRNA is initially distributed throughout the cytoplasm of unfertilized eggs ... The Macho-1 mRNA encodes a zinc finger DNA-binding protein that is believed to ...
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The coding region begins with the initiation codon, which is normally ATG. It ends with one of three termination codons: TAA, TAG or TGA. mRNA. Function of UTRs ...
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tRNA (transfer) - carriers that match amino acids to codons during translation ... mRNA has the codons. tRNA's deliver the amino acids and match anticodon to codon ...
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Rat mitochondrial IF1 protein mRNA, L07806, 883 bp. Rat casein kinase II alpha ... Rat mitochondrial succinyl-CoA synthetase alpha subunit J03621, 1684 bp ...
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