Protein Micropatterns Using a pHResponsive Polymer and Light - PowerPoint PPT Presentation
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Protein Micropatterns Using a pHResponsive Polymer and Light
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Protein Micropatterns Using a pH-Responsive Polymer and Light ... on surfaces using a pH-responsive polymer film and a ... Mask. Streptavidin. Scale bar = 25 m ... – PowerPoint PPT presentation
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Title: Protein Micropatterns Using a pHResponsive Polymer and Light
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Protein Micropatterns Using a pH-Responsive
Polymer and Light Karen L. Christman and Heather
D. Maynard, University of California, Los
Angeles DMI-0327077 through SINAM
The protein streptavidin (SA) binds strongly to
the small molecule biotin (vitamin H). Because
SA has four binding sites for biotin, it can be
used to as a linker between biotinylated
molecules in solution and biotins on a surface.
We have demonstrated patterning of SA on surfaces
using a pH-responsive polymer film and a
photoacid generator (PAG). Upon exposure of the
film (1) to deep UV light through a mask, the
polymer was chemically converted to reactive
aldehyde groups (1-2). Biotin was conjugated to
the surface (3), followed by SA (4) forming 18 x
18 mm feature sizes.
Christman, K. L. Maynard, H. D. Langmuir 2005,
21, 8389-8393.
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