Presentacin de PowerPoint

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BIOTOOLS BM LABS THE REAL TIME COMPANY New
York, 8th of November, 2006
www.biotools.net
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Biotools is a leading innovator and provider of
solutions for the diagnostic sector

• Spanish Biotech company with more than 10 years
  experience in the Molecular Biology field with
  innovating solutions in the diagnostic field
• Among our main shareholders are INIBSA S.A.
  (pharmaceutical company) and NAJETI (European
  venture capital company)
• Sales in 40 countries (Europe, Asia, Latin
  America and North America) with facilities in
  Spain (Headquarters) and Brazil
• We have the ISO 90012000 Certification as well
  as the Manufacturing License issued by the
  Spanish Health Ministry for our facilities, which
  enables us to develop and produce IVD Kits and
  reagents according to European Law (CE-Mark)

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Our Mission Development of innovating products
for the diagnosticbiomedical sector
Development of innovating systems and kits based
upon our OWN molecular biology technologies

• BIOMEDICAL RD and GENETICAL ENGINEERING
• DIAGNOSTICS, MONITORISATION MEDICAL SCREENING
• AGFOOD ENVIRONMENTAL CONTROL
• LEADERS in NEW TECHNOLOGY PLATFORMS for REAL
  TIME PCR

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Our Vision To be the leading referent for EASY
TO USE ONE STEP REAL TIME KITS

• Position Biotools in the next three years as the
  referent European company in the development of
  Ready to Use stabilized ONE STEP KITS for Real
  Time PCR based upon our GELIFICATION TECHNOLOGY
• Become global referent for the development of
  diagnostic kits and detection systems based upon
  Genomic and Proteomic technology
• Enter the US with a local partner offering the
  US market innovative, high quality and cost
  competitive solutions to actual demands in the
  diagnostics and RD markets based upon our own
  Technology platforms (GELIFICATION and
  LIONPROBES)

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Biotools has an extensive product portfolio
combining high quality with competitive prices

• RECOMBINANT THERMOSTABLE ENZYMES (based on
  Biotools own technology) for amplification of
  nucleic acids for standard and Real Time PCR, as
  well as supporting reagents
• Diagnostic standard and Real Time DNA
  amplification kits based upon our own patented
  LIONPROBES and GELIFICATION TECHNOLOGY
  PLATFORMS Tuberculosis, CMV, Malaria,
  Leishmania, Legionella, etc.
• Real Time and standard DNA amplification kits
  for Agfood and environmental controls GMO
  quantification kits, Listeria, Species
  Identification kits, etc.

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BIOTOOLS TECHNOLOGY PLATFORM

• LIONPROBES
• GELIFICATION TECHNOLOGY PLATFORM
• COMPUTERIZED MICROSCOPICAL HIGH-THROUGHPUT
  SCREENING

Technology patented and protected by Biotools BM
Labs,S.A.
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LIONPROBES TECHNOLOGY Overview
Innovating Real Time DNA Amplification technology
developed and patented by Biotools that has
offered us an alternative to the costly TaqMan
platform, blocked by Roche Basics of the system

• Dual marked oligoucleotide
• with fluorophore and quencher dyes at separate
  extremes

• A DNA polymerase with 3 5 exonuclease
  proofreading activity

Technology patented and protected by Biotools BM
Labs,S.A.
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LIONPROBES Market Advantages

• LIONPROBES are easily synthesized, and can be
  applied on existing Real Time equipment similar
  to TaqManTM probes, patented by Roche

• The LIONPROBESTM detection system is the
  property of BIOTOOLS, so that we do not have to
  rely on expensive licenses
• Combined with our extremely low cost enzyme
  production system, these technologies allow us to
  compete with high quality products in low cost
  emerging markets with huge sales volumes (China,
  India, etc.)
• Potential technology transfer to large and
  midsize diagnostic companies that are blocked by
  TaqMan patent

Technology patented and protected by Biotools BM
Labs,S.A.
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Proof of concept BIOTUB QT, an innovative kit
for a growing diagnostics problem
BIOTUB QT KIT
Kit for the quantitative detection of
Mycobacterium tuberculosis in clinical samples.
The detection methodology includes LIONPROBESTM
Figure Analysis of the obtained fluorescent
curves. A) FAM Channel using positive controls
(5x 101- 5x 105 copies/reaction) provided in the
BIOTUB QT Kit. B) Reading Channel for the
internal control included in the samples
Technology patented and protected by Biotools
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GELIFICATION PLATFORM MAKING DIAGNOSTICS EASY
and FAST
THE CORE TECHNOLOGY
Reaction mixes, containing all necessary reagents
including enzymes, primers, etc, are stabilised
for long time periods by the addition of
gelifying and stabilising agents and desiccation
at room temperature
Gelifying agent
Stabilising agent
ENZYME Thermostable polimerases (Taq or Tth) Pfu
polymerase Tth or Taq/Pfu
OTHER REAGENTS Primers (biotin- or
fluorescent-labeled) Nucleotides Buffers
Technology patented and protected by Biotools
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COMPUTERIZED MICROSCOPICAL SCREENING innovating
diagnostics
Basics of the system

• Computerized High-Throughput microscope for
  screening of complete swabs or tissue screening
  based on Support Vector Machine (SVM) without the
  requirement of the direct presence of a
  technician
• Successful tests being carried out for early
  stage diagnosis of oral cancer (swabs samples)
  and Tuberculosis (sputum samples) detection
• Objective is to expand the system to the
  diagnostic of other types of tumours to offer a
  fast, precise, cost competitive and standardized
  diagnostic method

Technology patented and protected by Biotools BM
Labs,S.A.
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What can Biotools offer?

• Innovative, high-quality and price competitive
  product portfolio
• with important applications in the major and
  expanding markets
• in the US and Canada (Diagnostics, Pharma and
  RD)
• A strong IP, patent portfolio and applied
  technologies that are
• prone to provide BIOTOOLS with a major
  competitive advantage
• in the diagnostics market (Computerized
  Microscope Platform)
• 10 years of global experience in the diagnostics
  and the RD
• sector
• A business-oriented mentality, flexibility and
  devotion to convert
• science into products that provide optimal,
  high quality and cost
• competitive solutions