LAB SAFETY

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LAB SAFETY
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Apparel

• Shoes
• Lab coats
• Safety glasses

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Note!

• No eating, drinking, or smoking in the lab at any
  time.

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Spills

• Wash
• Notify
• Receive instructions

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Accidents

• Be careful!
• Pay attention
• Report any injuries

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Other Matters

• Broken glass
• Other waste
• Pipetting
• Volatile chemicals
• Dirty labware
• Labeling
• General cleanliness

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ASEPTIC TECHNIQUE
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I. Contaminants

• A. Bacteria, fungi, and insects
• B. Initial contaminants
• C. Latent or persistent contamination
• D. Introduced contamination
• E. Detection of contaminants

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THE TRANSFER HOOD

• The hood should remain on
• Necessary items present, unnecessary things
  removed
• Check the bottom of the hood for blockage
• Wash hands

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THE TRANSFER HOOD

• Spray or wipe the inside of the transfer hood
• Wipe hands and lower arms with 70 EtOH
• Remove dangling sleeves and jewelry

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Sterilization and use of supplies and equipment

• Know which of your implements, flasks, etc. are
  sterile and which are not
• Monitor autoclave time
• Sterilized items should be used within a short
  time
• Items that come packaged sterile should be
  examined carefully for damage before use

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Sterilizing tools, vessels etc.

• Autoclaving
• Ethylene oxide gas
• UV radiation
• Dry heat
• Microwave

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Working in the transfer hood

• Work well back in the transfer hood
• Make sure that materials in use are to the side
  of your work area, so that airflow from the hood
  is not blocked
• Instruments (scalpels, forceps) can be sterilized
  by flaming or immersion in hot beads

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Working in the transfer hood

• Plant material should be placed on a sterile
  surface when manipulating it in the hood
• Remove items from the hood as soon as they are no
  longer needed
• When transferring plant cultures, do contaminated
  cultures last.

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Working in the transfer hood

• When finished in the hood, clean up after
  yourself.
• Be sure when you are finished that you turn off
  the gas to the burner
• It is pointless to practice good sterile
  technique in a dirty lab

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Surface-sterilizing plant material

• Care of stock plants
• Alcohol
• Bleach
• Calcium hypochlorite
• Mercuric chloride
• Hydrogen peroxide
• Rinsing

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In the media

• Use of antibiotics and fungicides
• Plant Preservative Mixture
• Rinsing