DNATRACK

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DNA-TRACK OLIV-TRACK

• Coordinator
• Nelson MARMIROLI
• University of Parma - ITALY

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Aim and objective of DNA-TRACK

• The aim of the DNA-TRACK project was to develop
  scientific and socio-economical measures to
  detect DNA of alien origin (including DNA from
  GMOs) at different levels in the food chain.
• This goal has been pursued with a completely new
  scientific and technical approach based on PCR
  techniques assisted by complementary microarray
  and PNA technologies.

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Aim and objective of OLIV-TRACK

• The main objective of this project was to apply
  molecular technologies based on genomic and
  metabolic information to the traceability of
  origin and authenticity of olive oil produced and
  sold within the European Union. The purpose of
  this is to ensure the production of olive oil of
  reliable quality for the consumers' health and
  confidence, and to protect sustainable
  cultivation of olive trees to authenticate their
  European region of origin.
• Preservation of the quality and of the identity
  of the blend utilised in oil production requires
  an analytical approach through the entire
  production and transformation chain.

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Traceability aspects

• FOOD GENOMICS
• Food genomics is the identification and
  classification of parts of genetic information
  belonging to animals, plants and microorganisms
  used in preparation or production of food
• It has the purpose of ascertaining if the
  composition or the preparation of the food
  product is in agreement with a technical
  specification (PDO, PGI), with a label or with a
  whole procedure (traceability)

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METABOLOMICS
analysis
Metabolite extraction
Classification of oils into groups based on
chemical composition and determination of
geographic origin
Food products
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GENOMICS
analysis
Extraction of molecules
Identification of ingredients through approaches
based on molecular markers
Food products
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Impact

• Methods for DNA extraction from different
  matrices
• Identification of molecular markers and DNA
  fragments useful for traceability (AFLP, SNP,
  SSR)
• Development and validation of protocols and
  strategies for qualitative and quantitative PCR
  analyses, uniplex and multiplex
• Development of PNA clamping protocols
• Development of microarray systems
• Metabolomic analyses and parameters useful for
  determination of geographic origin

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Interaction with other projects

• The strategies, methods and protocols are
  currently applied in projects CO-EXTRA and CHILL
  ON
• They have also brought to projects at the
  National level, financed by Italian Ministries
  and by the Region Emilia-Romagna

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Interaction with stakeholders

• The partnerships involved companies, consumer
  associations, associations of producers
• The dissemination activities have addressed
  several types of stakeholders

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Dissemination activities

• WWW sites of the projects
• Leaflets
• Press releases and articles on newspapers and
  magazines
• Scientific publications and books
• Participation to congresses of National and
  International societies with oral presentations
  and posters
• Participation to cultural events directed to the
  general public
• Participation to specialised conferences,
  exhibitions

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Faculty of Health Sciences
Panum Instituttet
Traceability of DNA fragments throughout the
food chain by DNA/PNA technologies. Application
to NOVEL FOODS. EU Project Contract
NQLK1-2000-01658 Coordinator Prof. Nelson
Marmiroli, University of Parma, IT
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Quality of Life and Management of Living Resources
QLK1-CT-2002-02386
Traceability of origin and authenticity of olive
oil by combined genomic and metabolomic approaches
Coordinator Nelson Marmiroli University of
Parma Parma, Italy
www.dsa.unipr.it/foodhealth