LAB SAFETY

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LAB SAFETY
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Lab Safety

• Proper handling of equipment and chemicals
• Prevents harm to self, others and property

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WHAT IS IT?

• THE PRACTICE OF HANDLING EQUIPMENT AND CHEMICALS
  ACCORDING TO SPECIFIC METHODS FOR THE PURPOSE OF
  PREVENTING HARM TO YOURSELF, OTHERS AND PROPERTY.

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What is it?

• Proper handling of equipment and chemicals
• Prevents harm to yourself, others, property

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BASIC LAB PRINCIPLES

• WEAR PERSONAL PROTECTIVE EQUIPMENT
• KEEP AREA CLEAR
• NO FOOD OR DRINK
• USE EYE WASH SAFETY SHOWER
• MATERIAL SAFETY DATA SHEETS

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PERSONAL PROTECTIVE EQUIPMENT (PPE)

• SAFETY GLASSES (PROTECTS EYES)
• GLOVES (NOT ALL GLOVES ARE THE SAME) (PROTECTS
  HANDS)
• LAB COAT OR LONG SLEEVE SHIRT (PROTECTS ARMS AND
  TORSO)
• LONG PANTS (PROTECTS LEGS)
• STURDY SHOES (NO FLIP-FLOPS OR SANDALS) (PROTECTS
  FEET)

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Personal Protective Equipment (PPE)

• Safety glasses
• Gloves
• Lab coat/long-sleeved shirt
• Long pants
• Sturdy shoes

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PROTECTS FROM WHAT?

• Chemical spills
• CORROSIVE (PH lt 2 OR gt12)
• HOT LIQUIDS
• BIOHAZARDS

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KEEP AREA CLEAR CLEAN

• REMOVE ALL BACKPACKS, COATS ETC. FROM WORK AREA
  TO PREVENT TRIPS FALLS
• CLEAN UP ALL SPILLS, PAPER SCRAPS, AND GLASSWARE
  TO PREVENT CUTS, BURNS AND FIRE
• LAB INSTRUCTOR WILL GIVE SPECIFIC INSTRUCTIONS
  FOR CLEANING UP SPILLS

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Keep Area Clear and Clean

• No backpacks, obstructive materials
• Clean up glassware, paper and spills
• Consult instructor on chemical spill clean-up

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FOOD DRINK

• NO FOOD OR DRINK IS ALLOWED IN THE LABORATORY
• NO FOOD OR DRINK IS TO BE STORED IN LAB
  REFRIGERATORS

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Food Drink

• No food or drink in laboratories or lab
  refrigerators

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EYE WASH

• IF YOU GET CHEMICALS IN YOUR EYES FLUSH WITH
  WATER FOR 15 MINUTES AND SEEK MEDICAL ATTENTION.
  CALL 811.
• LOCATED AT LAB SINK

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Eye wash

• Flush eyes with water for 15 mins
• Seek medical attention
• Call 811

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SAFETY SHOWER

• IF YOU SPLASH CHEMICALS ON YOURSELF GO TO THE
  SAFETY SHOWER AND RINSE YOURSELF FOR 15 MINUTES.
  CALL 811.
• LOCATED IN LAB OR IN HALL OUTSIDE LAB

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Safety shower

• If you spill chemicals on yourself
• Rinse off body part for 15 mins
• Seek medical attention
• Call 811

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PROPER METHODS FOR USING.GLASSWARE

• MAKE SURE GLASSWARE IS NOT CRACKED
• LABEL ALL BEAKERS, FLASKS, TEST TUBES, ETC. WITH
  WHAT IS IN THEM.

TOULENE
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GLASSWARE-CONT

• CLEAN ALL GLASSWARE USING A LABORATORY DETERGENT
  (ALCONOX) AND TAP WATER. RINSE SEVERAL TIMES AND
  FOR FINAL RINSE USE A SMALL AMOUNT OF DEIONIZED
  WATER.
• THE GLASSWARE IS CLEAN IF NO WATER DROPLETS
  ADHERE TO THE CLEAN PART OF THE GLASSWARE
• NEVER DISPOSE OF BROKEN GLASS IN THE TRASH. PLACE
  IN BROKEN GLASS BOX

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Glassware

• Use detergent and water to clean glassware
• Use deionized water for last rinse
• Dispose broken glass in separate container

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USING CHEMICALS

• READ CONTAINER LABEL AND MSDS BEFORE USING
• IMPORTANT-DO NOT PUT EXCESS CHEMICALS BACK INTO
  BOTTLE. YOU CAN CONTAMINATE THE CHEMICAL POSSIBLY
  CAUSING A BAD REACTION THE NEXT TIME THE CHEMICAL
  IS USED.

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Using chemicals

• Read MSDS and container label before using
• Dispose of all excess chemicals
• Prevent chemical contamination

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CHEMICALS-DISPOSAL

• DO NOT POUR CHEMICALS DOWN THE DRAIN-PLACE IN
  SPECIFIC WASTE CONTAINERS.
• EPA AND THE CITY OF DES MOINES HAVE SPECIFIC
  REGULATIONS REGARDING THE DISPOSAL OF LABORATORY
  CHEMICALS. (Criminal penalties and big fines if
  you do it wrong)

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WORKING IN FUME HOOD

• CHECK AIR FLOW BY TAPING A PIECE OF PAPER TO THE
  OUTSIDE OF THE FUME HOOD SASH. THE PAPER SHOULD
  BE PULLED TOWARD THE INSIDE OF THE HOOD. BE
  CAREFUL NOT TO HAVE PAPER PULLED INTO HOOD FAN AS
  IT CLOGS THE DUCTS.

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FUME HOODS CONT

• USE THE HOOD WHEN WORKING WITH HAZARDOUS
  CHEMICALS TO PREVENT INHALATION OF FUMES THAT MAY
  HARM YOU
• CLOSE SASH AS MUCH AS POSSIBLE
• TO PREVENT INJURY IN CASE OF AN EXPLOSION.

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NEVER WORK ALONE IN THE LAB

• WHAT CAN HAPPEN ?

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MATERIAL SAFETY DATA SHEETS

• READ BEFORE USING A CHEMICAL
• THEY GIVE YOU IMPORTANT INFORMATION ABOUT THE
  USE, HAZARDS AND DISPOSAL OF THE CHEMICAL
• LOCATED IN LAB AND ON INTERNET

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MSDS CONT

• MSDSs PROVIDE THE FOLLOWING INFORMATION
• Mfg. Name
• Emergency Contact Info
• Product Name
• Hazardous Ingredients
• Physical Properties
• Fire Fighting info.

• Reactivity information
• Personal Protective equip.
• Waste disposal info.
• Environmental/
• Regulatory Info

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