ETIQUETTE

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ETIQUETTE

• WHAT IS ETIQUETTE?

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ETIQUETTE

• Manners (good manners)
• Protocol (procedure, code of behavior) custom
• Decorum (modesty, good behavior)
• propriety (politeness, modesty)

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LABORATORY ETIQUETTE AND PROTOCOL

• You will be working with various types of
  chemicals and microorganisms, and operating a
  wide array of laboratory equipment.
• How you treat your workspace and instruments will
  have a direct impact on your experiments.

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LAB ETIQUETTE

• IT SHOULD BE A PRODUCTIVE DISCOVERY,DEVELOPMENT,
  DESIGNING, COLLABORATING AND GENERAL LEARNING
  PLACE.
• IT REQUIRES THAT WE ALL HELP,COOPERATE TO
  MAINTAIN A RESPECTFUL AND SUPPORTIVE ENVIRONMENT

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LAB ETIQUETTE PRINCIPLES

• RESPECT OTHERS PROJECTS AS YOU WOULD EXPECT
  OTHERS TO RESPECT YOURS.
• MAINTAIN A PROFESSIONAL ENVIRONMENT,
  PROFESSIONAL TECHNIQUES, PROFESSIONAL ATTITUDE
  TOWARDS OTHERS.
• TAKE RESPONSIBILITY FOR THE FACILITY AND YOUR
  COLLEAGUES
• USE MODERN TECHINIQUES FOR THE RESEARCH
  (COMPUTERS, PRINTERS)

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ETIQUETTE IN LABORATORY

• ARRIVAL IN THE LABORATORY
• Students staff need to come in time
• Delay has to be avoided teacher must give proper
  warning
• avoid display of excess enthusiasm or emotion
• ARRANGEMENT OF THE LABORATORY
• Number of prepared task called experiments
• Each has a place and specific task or
  experiment or name
• Discover which experiment for the day
• Plan the chart , seat yourself on a stool
• Apparatus - substantial, well fashioned from
  metal or timber,

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ETIQUETTE IN LABORATORY

• DISCOVER THE NATURE OF THE TASK
• EACH EXPERIMENT WILL REQUIRE THE PERFORMANCE OF
  A NUMBER OF DEFINITE AND PREDETERMINED OPERATION
• FIRST DISCOVER THE TASK, KNOW YOUR SELF BETTER,
  DONT FEEL SOMETHING IS MISSING. INCLUDING THE
  APPARATUS AND KNOW THEM WELL
• SCRIPT
• TEACHERS JOB KNOWLEDGABLE, GOOD CONTENT
  KNOWLEDGE THEORY AND PRACTICAL, GOOD
  EXPRESSION, SHOULD BE CLEAR AND POLITE .
• WE ARE NOT DISCOVERING, WE ARE FOLLOWING

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ETIQUETTE IN LABORATORY

• CONDUCT OF EXPERIMENT
• CAREFULLY STUDY THE NOTES
• CONSULT YOUR LABORATORY NOTEBOOK THROUGHOUT THE
  EXPERIMENT
• CONSTANTLY PAY ATTENTION TO THE WORK YOU ARE
  DOING
• ENSURE YOUR PERFORMANCE IS CORRECT AT EVERY STAGE
• DO NOT BE LETHARGIC OR IMPATIENT
• BE CONFIDENT BUT DONT EXPECT THE RESULT THAT YOU
  WANT

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LABORATORY ETIQUETTE

• LABORATORY NOTE BOOK
• MAINTAIN SEPARATE BOOK FOR EACH SECTION OF
  EXPERIMENT (BIOCHEMISTRY, HAEMOTOLOGY)
• THIS IS NOT SPECULATION, DONT WRITE ERRONEOUS
  THOUGHTS
• A TIDY RECORD DEMONSTRATES WHAT YOU OUGHT TO
  HAVE DONE
• ADVANTAGE LIES IN HAVING ARRIVED AT AN ANSWER
  CLOSE TO, BUT NOT IDENTIFIED WITH,
• IT IS A RECORD BOOK .

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LABORATORY ETIQUETTE

• SOCIAL INTERCOURSE IN THE LABORATORY
• IT IS NOT PLACE FOR INTIMACY
• AVOID GENERAL CONVERSATION
• TEACHER NEEDS TO FOCUS ON ADEQUACY OF TESTING
  PRODEEDURES EXPERIMENT
• TEACHERS AND STUDENTS NEED TO AVOID ANY STRONG
  EXPRESSION OF FEELING
• CRITICISM BY A TEACHER SHOULD BE MILD
• STUDENT SHOULD ACCEPT THE TEACHERS JUDGEMENT
  WITHOUT ARGUMENT

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LABORATORY ETIQUETTE

• IMPORTANCE OF THE LABORATORY
• NOT ATTENDING THE LABORATORY IS THE FAULT (STOOL
  EXAMINATION,URINE EXAMINATION)
• IT IS UNIVERSALLY ACKNOWLEDGED TO BE ESSENTIAL

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LAB SAFETY

• Please Note that there are three primary routes
  of entry into the body
• (i) Ingestion (swallowing)(ii) Absorption
  (contact with eye and skin may be absorbed into
  the body or cause local effects)(iii) Inhalation
  (breathing in)

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LABORATORY SAFETY ETIQUETTE

• When in the lab, you must always wear safety
  glasses/goggles, long pants, a laboratory coat,
  and closed-toed shoes (i.e., no sandals)

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LABORATORY ETIQUETTE AND PROTOCOL

• Know the location of safety showers, eyewash
  stations, and the first aid kit.

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LABORATORY ETIQUETTE AND PROTOCOL

• Label everything - All flasks/bottles/etc. must
  be labeled with their contents, your
• name, and the date you made it. This even goes
  for a flask of water (you are the only one who
  knows that its only water!).
• Keep your workspace uncluttered. Cluttered
  workspaces lead to confusion and spills.

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LABORATORY ETIQUETTE AND PROTOCOL

• Make sure to clean your bench top
• CHEMICALS RESIDUES WITH BENCH BRUSH
• EXPERIEMENT WITH BACTERIA - WIPE THE AREA
  ETHANOL
• HYDROPHOBIC CHEMICALS - WIPE THE AREA WITH
  METHANOL
• After each use, this must be done.

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LABORATORY ETIQUETTE AND PROTOCOL

• Wipe the sink area after each use.

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LABORATORY ETIQUETTE AND PROTOCOL

• Wash all glassware immediately after use
• Put away the glassware once it is dry.

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LABORATORY ETIQUETTE AND PROTOCOL

• Place an order before the item runs out
• Read the manual of each instrument you use and
  know how to use,

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LABORATORY ETIQUETTE AND PROTOCOL

• Pipette Put them back on the pipette racks
• Balances The key point with balances is that
  you must clean the balance after every use, even
  if you dont visually see any chemical residue.
• pH meters Treat pH meters with care. pH meters
  and probes can be very delicate

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LABORATORY ETIQUETTE AND PROTOCOL

• Centrifuge Two key points are to
• always make sure all samples are balanced within
  the centrifuge and
• thoroughly clean the centrifuge after use if a
  tube either leaks or breaks during use.

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LABORATORY ETIQUETTE AND PROTOCOL

• MINIMIZING CONTAMINATION
• There are many opportunities for contamination to
  occur in the laboratory.
• Improper actions resulting in contamination will
  not only affect your experiments, but the
  contamination can also affect your lab mates
  experiments.

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SPILL KIT

• A SPILL KIT IS A COLLECTION OF EQUIPMENT AND
  MATERIAL THAT CAN BE USED TO CLEAN UP SPILLS OR
  LEAKS OF HAZARDOUS MATERIAL.
• THE CONTENT OF THE SPILL KITS MUST BE MATCHED TO
  THE TYPES OF HAZARDOUS MATERIALS THAT COULD
  OCCUR.

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SPILL KIT

• SPILL-X-A USED TO NEUTRALIZE ACIDS ( NITRIC
  ACID, HYDORCHLORIC ACID)
• SPILL-X-C- NEUTRALIZE BASE (NAOH)
• SPILL-X-S-VOLATILE ORGANIC CHEMICALS LIKE ACETONE
• SPILL-X-FP-NEUTRALIZE FORMALDEHYDE

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LABORATORY ETIQUETTE AND PROTOCOL

• EXPERIMENTAL PLAN
• Set-up the workspace
• Prepare solutions and glassware
• Run the experiment
• Clean-up workspace
• Dispose of hazardous and biological wastes
• Wash and store glassware

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LABORATORY ETIQUETTE AND PROTOCOL

• Dont underestimate the importance of this.
• Postponing the clean-up not only creates safety
  and contamination issues, but it will also impact
  the functioning of the laboratory

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LAB SAFETY

• Even in "tracer" amounts they may constitute a
  health hazard. The health hazard arises in the
  following way (1) from external sources
  emitting radiations(2) accidental ingestion by
  swallowing or by inhalation of gases, vapours,
  dust, or powder and(3) by contamination of the
  person (skin, clothing, hair).

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LAB SAFETY AND ETIQUETTE

• You may not eat or drink in any of the
  laboratories. There are simply too many poisonous
  and explosive substances around

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LAB SAFETY AND ETIQUETTE

• Please keep in mind that laboratory is a
  "smoke-free" zone smoking is forbidden in ALL
  rooms.
• It is, in fact, illegal under the industrial
  safety regulation to which we are subject.

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LAB SAFETY AND ETIQUETTE

• Rigorous cleanliness throughout all of your
  procedures.
• This also reduces the risk of contamination of
  the apparatus (balances, microscopes,
  centrifuges, special glassware, etc.).

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LAB SAFETY AND ETIQUETTE

• Special care must be taken with many of the
  chemicals used in labs. Some are extremely
  dangerous poisons can do lasting damage if
  accidentally ingested, splashed in the eyes, or
  simply inhaled as fumes in too great quantities
  -- here formalin is the most obvious example.

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LAB SAFETY AND ETIQUETTE

• This is particularly important if you will be
  handling specimens preserved in formalin.
• If you splash chemicals in your eye, flush
  IMMEDIATELY for at least 20 minutes at the
  eyewash stations

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EYE SHOWER
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LAB SAFETY AND DISPOSAL

• Be extremely careful about disposing of sharp or
  otherwise dangerous items. OLD SCALPEL BLADES,
  HYPODERMIC NEEDLES, AND THE LIKE, MUST BE PLACED
  IN THE YELLOW SHARPS CONTAINERS AND BROKEN GLASS
  MUST BE PLACED IN THE BROKEN-GLASS CONTAINERS (20
  LITER BUCKETS), not in the waste paper
  containers. A person may get hurt while handling
  it.

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LAB SAFETY AND DISPOSAL

• NEVER REUSE SCALPEL BLADES, HYPODERMIC NEEDLES,
  AND THE LIKE. IT MAY SPREAD DANGEROUS DISEASES.

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LAB SAFETY AND ETIQUETTE

• If handling living things (animal or insect ),
  they must always be handled with care

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LAB ETIQUETTE

• A laboratory should be and can be a productive
  discovery, development, designing, collaborating,
  and general learning place.
• This can happen if everyone cooperates to
  maintain a respectful and supportive environment.
  
•  

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LAB ETIQUETTE

• Suggest supplies and equipment that would be
  beneficial to improve the facility

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LAB ETIQUETTE

• If you are not committed to being a part of the
  solution, you are likely part of the problem.

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ETHICS AND ETIQUETTE IN LABORATORY

• THANK YOU
• Dr.M.Prakash Kumar M.D.(Hom)
• Professor
• Government Homoeopathic Medical College
• Bangalore -560079