Small Diameter Centrifuge Tube

1
Small Diameter Centrifuge Tube

• Team Members
• Sarajane Stevens - Team Leader
• Anita Zarebi - Communicator
• Christopher Westphal - BWIG
• Katy Reed - BSAC

Advisor Professor Kristyn Masters
Client Pamela Kling, M.D.
2
Presentation Outline

• Problem Statement
• Background Information
• Research Competition
• Suggested Materials
• Design Constraints
• Alternative Designs
• Future Work

3
Problem Statement

• Client Pamela J. Kling, M.D.
• Blood samples
• Small Diameter Centrifuge Tube
• Super clear, strong plastic
• Large lengthdiameter ratio
• Volume 70µl
• Adaptor for centrifuge

4
Background Information

• Anemia in newborns
• Red blood cell deficiency
• Drug research
• Multiple, consistent samples
• Reticulocytes
• Immature red blood cells
• Stimulates cell production
• Analyzed for iron

5
Background Information
6
Current Devices Centrifuge Tubes

• Typical blood sample 10ml
• Smaller volumes
• Small lengthdiameter ratio
• Material deforms in floor model centrifuge

7
Research Competition

• Private research companies
• Techniques, products for centrifuge systems
• Direct use
• Beckman-Coulter
• Fisher Scientific
• Eppendorf

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Suggested Materials

• Polyethylene
• Deforms in floor model
• Cloudy
• Polypropylene
• Strong
• Clear
• Tygon tubing
• Cut to desired length
• Superclear plastics
• Polycarbonate

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Design Constraints

• Super clear plastic
• Volume
• LengthDiameter ratio 501
• 1 hour long centrifugation
• 15,000-37,000 x g
• Temperature 30-40C

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Problem Overview

• Separation
• Trial Error Options
• Microhematocrit centrifuge
• Ultracentrifuge
• Materials
• Tube choice

• Extraction
• Seal and Cut
• Removal by pipet
• Titration method

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Design I Seal and Cut

• Critoseal
• Hematocrit tube cutter
• Collection container or syringe

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Design I Pros and Cons

• Pros
• Quick and easy
• Increases purity of sample
• Utilizes current lab equipment

• Cons
• Use more Critoseal
• Remnants of tubes create untidiness

Critoseal
Critoseal
Whole Blood
Reticulocytes
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Design II Simple Extraction
Syringes/Pipette

• Narrow tubes
• Tube holder
• Magnifying glass

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Design II Pros and Cons
Syringe/Pipette

• Cons
• Syringe
• Inconvenient withdrawal
• Pipette
• One tube at a time

• Pros
• Syringe/Pipette
• Variable volume
• No vacuum
• Syringe
• Multiple in one syringe
• Pipette
• One hand operation

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Design III Titration
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Design III Pros and Cons

• Cons
• Direct contact with sample--gloved hand
• Multiple tests--time consuming
• Tubes may break upon slicing

• Pros
• Eliminates suction
• Ability to stop flow early
• Isolate/pipet easily

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Future Work

• Test all methods--combine?
• Plastics research
• Temperature tolerance
• High g tolerance
• Construct AND test prototype
• Consult client

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References

• 1 General Use Needles. 15 Feb 2005.
  http//catalog.bd.com/bdCat/listProducts.doCustome
  r?categoryID134priority25categoryNameGeneral
  20Use20NeedlescategoryImgPath/ecat/images/f07/f
  amily/needles.jpgsortByFieldProduct20Numbervie
  wPageNum0
• 2 GELoader Tips. 13 Feb 2005.
  http//www.eppendorf.com/script/cat-prd.php?l25r
  egionb9734
• 3 Diabetes Dictionary. 13 Feb 2005.
  http//diabetes.niddk.nih.gov/dm/pubs/dictionary/P
  -T.htm
• 4 Murphy, J.R. 1973. Influence of temperature
  and method of centrifugation on the separation of
  erythrocytes. Journal of Laboratory Clinical
  Medicine 82334-341
• 5 Brugnara, C. et al. 1993. Red blood cell
  regeneration induced by subcutaneous recombinant
  erythropoietin iron-deficient erythropoiesis in
  iron-replete subjects. Blood. 1993 81(4)
  956-964
• 6 SuperClear Microcentrifuge Tubes. 17 Feb
  2005. http//www.labdepotinc.com/product_detailsi
  d52pid150.aspx
• 7 VisiPak Extrusions. 17 Feb 2005.
  http//www.visipak-extrusion.com/
• 8 Fisherbrand Plastic Microhematocrit Tubes. 17
  Feb 2005. https//www1.fishersci.com/Coupon?gid17
  8612cid1333
• 9 Hematology and Immunology Studies. 17 Feb
  2005. http//history.nasa.gov/SP-368/p212.jpg
• 10 Axeda. 11 Feb 2005. http//www.axeda.com/imag
  es/customers/BeckmanCoulter_logo.jpg
• 11 Chem Lab Cricket Team. 13 Feb 2005.
  http//www-leeper.ch.cam.ac.uk/Fisher2.jpg
• 12 BioBank. 12 Feb 2005. http//www.biobank.co.k
  r/maker/eee/eppendorf-logo.gif

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Questions?