PowerPointPrsentation

1
Institut für Biochemie und Molekulare Medizin
Lecture 2 Mechanism of initiation Michael
Altmann WS 2007/2008
2
Initiation
Cap-dependent initiation
Re-initiation
Eukaryotic translation H. Trachsel
3
Initiation
Internal initiation
Ribosome shunting
Eukaryotic translation H. Trachsel
4
The pioneers
Max Schreier
Theophil Staehelin
Eukaryotic translation H. Trachsel
5
Eukaryotic translation H. Trachsel
The Schreier-Stähelin-System
6
(No Transcript)
7
Translation initiation factors
Eukaryotic translation H. Trachsel
8
Model of initiation
Eukaryotic translation H. Trachsel
9
Protein-protein interactions
Reference
Eukaryotic translation H. Trachsel
Kapp LD Lorsch JR
Ann. Rev. Biochem. 73 657-704, 2004
10
Multi-factor complex (MFC)
Reference
KH Nielsen et al.
Eukaryotic translation H. Trachsel
Mol. Cell. Biol. 26 2984-2998, 2006
11
Cap-eIF4E-eIF4G interaction
Reference
T von der Haar et al.
Eukaryotic translation H. Trachsel
J. Mol Biol. 356 982-992, 2006
12
Initiation complex
Reference B. Siridechadilok et al. Science 310
1513-1515, 2005
Eukaryotic translation H. Trachsel
13
RNA helicase
Eukaryotic translation H. Trachsel
14
DEAD helicase
Reference
Eukaryotic translation H. Trachsel
T Sengoku et al.
Cell 125 287-300, 2006
15
Eukaryotic translation H. Trachsel
16
AUG recognition
Reference
Eukaryotic translation H. Trachsel
AM Cigan et al.
Science 242 93-96, 1988
17
AUG recognition
Reference
Eukaryotic translation H. Trachsel
TF Donahue et al.
Cell 54 621-632, 1988
18
AUG recognition
Reference
Eukaryotic translation H. Trachsel
M Kozak
Gene 234 187-208, 1999.
19
Eukaryotic translation H. Trachsel
20
Internal initiation cricket paralysis virus
Referenz
Eukaryotic translation H. Trachsel
21
Seminar 1
- Explain to a layman how genes regulate biol.
processes!
- What are the biol. consequences of mRNA
transport and localized
translation?
- Inform yourself about CCA adding enzyme (tRNA)!
- How many rRNA genes does a cell need to make
106 ribosomes
in 6 hours?
- What are the most prominent differences in the
mechanism of
initiation between prokaryotes and eukaryotes?
- Are there orthologs of eIFs?
- How are protein-protein interactions measured?
- Why should a cell need so many helicases?
Eukaryotic translation H. Trachsel