Consumer Products Study

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Consumer Products Study

• Disinfectants, Mouthwash Antibacterial Hand Soap,
  Antibiotics, and Spices

 
 
By THE WESTON SCHOLARS!!!!! (and gifted and
talented students.)
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MATERIALS

• Tape
• Wax pencil
• Petri dish
• Cotton swabs
• Forceps
• Nutrient broth
• Sterile disk
• Inoculating wire loop
• Sterile water
• Bunsen burner
• Nutrient agar plate
• All consumer products

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Procedure
Preparing Nutrient Agar Plates

• Suspend 23g of nutrient agar powder in 1L
  purified H2O.
• Mix and heat solution until powder is dissolved
  and solution becomes an translucent yellow.
• Autoclave at 121ºC for 15 minutes.
• Fill as many petri dishes as possible (half full)
  with nutrient agar and let them solidify.

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Procedure (contd)

• Dip cotton swab in sterile H2O and take samples
  from mouth, skin, and environment.
• Rub the swab on nutrient agar plate.
• Cover nutrient agar plates and label them.
• Invert nutrient agar plates and place them into
  the incubator at 37ºC.

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Procedure (contd)

• For discontinuous streaking, sterilize wire loop
  by flaming until the wire turns bright orange.
• Cool loop by lightly touching the wire to the
  nutrient agar.
• Using the wire loop, take bacteria from the
  nutrient agar plate and streak 4 times on a new
  agar plate and flame the loop in between.
• Cover, label, and tape plates shut.
• Place, inverted, in the incubator at 37C.

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Discontinuous Streaking
 
 
 
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Procedure (contd)

• For the continuous streaking, use the same
  procedure as the discontinuous streaking, except
  you just streak back and forth until you cover
  the whole nutrient agar plate.

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Continuous Streaking
 
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Procedure (contd)

• Re-suspend bacteria
• 1. Pour 10ml of nutrient broth into the
  continuously streaked plates.
• 2. Swab bacteria until all bacteria is suspended
  in liquid
• 3. Swab bacteria into new plates.
• 4. Dip sterile disk in disinfectants,
  antibiotics, spices, mouthwash, or antibacterial
  hand soap and place in center of plates. Tap
  gently to secure.
• 5. Label plates and place them inverted in the
  incubator.
• 6. Let plates sit in the incubator over night.
• 7. Measure the zone of inhibition (in
  millimeters) and make your conclusions.

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Zone of Inhibition
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Disinfectants

• Ajax
• Algacide
• Americas Choice Pine Cleaner
• Ammonia
• 10 Bleach
• Clorox
• Fantastik-Clear
• Fantastik-Purple
• Scrubbin Bubbles
• Soft Scrub
• Spic and Span
• Tilex
• Touch of Glass
• Wavicide
• White Vinegar
• Windex
• Xtra Pine
• 409

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Disinfectants
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Results
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(No Transcript)
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Results
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Best Disinfectants

• 1

3
2
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Mouthwashes

• Biotene
• Cepacol
• CVS Brand
• Diluted Hydrogen Peroxide
• Listerine
• Listerine Tartar Control
• Plax
• Rite Aid Brand
• Salt Water
• Scope
• ShopRite Brand
• Toms Natural Mouthwash

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Results
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Results
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Best Mouthwashes

• 1

3
2
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Hand Soaps

• Bath Body Works
• Clean n Smooth
• Ajax
• Palmolive
• Soft Soap
• April Body Shower

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Results
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Results
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Best Hand Soaps
3

• 1

2
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Antibiotics on the skin bacteria

• Tetracycline
• Rifampin
• Chloramphenicol
• Kanamycin
• Streptomycin
• Triple Sulfonides
• Polymyxin B
• Erythomycin
• Cephalothin

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Results
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Results
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Best Antibiotics

• 1 Rifampin
• 2 Caphalothin
• 3 Chloramphenicol

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Spices

• Cinnamon
• Paprika
• Chili Powder
• Onion Powder
• Cream of Tartar
• Coriander
• Mustard Powder
• All Spice
• Alum
• Cumin
• Nutmeg
• Cajun Seasoning
• Curry
• Ground Ginger
• Garam Marsala
• Garlic

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Results
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Results
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Best Spices
3

• 1

2
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(No Transcript)
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Dr. Lee H. Lee
Thank you!
Pat Platner Shi-Fang Hsu Tina Chu Alex Peroff
Ms. English Ms. Portas
AND
Mr. Mrs. Weston