Liquid Chromatography

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Liquid Chromatography
Ulrich Tallarek and Ernst Bayer Institute of
Organic Chemistry University of
Tübingen Tübingen, Germany Dagmar van Dusschoten
and Henk Van As WnmrC
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Fluid Flow
moving fluid
stationary fluid
overall flow direction of fluid
internal pore
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Mass Transfer
stationary fluid (21) diffusion within
particles
Conditions - water flow rate 8.8 mL/min -
observation time 30 ms
moving fluid (79) dispersion between particles
Averaged Propagator a.u.
Dynamic Displacements m
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NMR Image of Column
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Transport Parameters

• Intra-particle diffusion and tortuosity
• Inter-particle dispersion and porosity
• Stagnant mobile phase mass transfer
• Spatial distribution in packed beds

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Relevance

• (Bio)chromatography, ion exchange, and adsorption
• Separation science, and technology
• Groundwater flow and contaminant dispersion
• Chemical engineering and bioreactor mass transfer
• Column operation and compression technology